Radiation hybrid comparative mapping between human chromosome 17 and porcine chromosome 12 demonstrates conservation of gene order
文献类型：期刊论文
| 作者 | Shi XW1,2; Fitzsimmons CJ1; Green JA4; Tuggle CK[*]1; Genet C3; Prather R4; Whitworth K4 |
| 刊名 | ANIMAL GENETICS
 |
| 出版日期 | 2001 |
| 卷号 | 32期号:4页码:205-209 |
| 关键词 | comparative map HSA 17 SSC12 pig radiation hybrid mapping |
| 通讯作者 | cktuggle@iastate.ed |
| 合作状况 | 其它 |
| 英文摘要 | A comparative study of human chromosome 17 (HSA17) and pig chromosome 12 (SSC12) was conducted using both somatic cell hybrid panel (SCHP) and radiation hybrid (RH) panel analysis. Sequences from an expressed sequence tag (EST) project in pig reproduction were examined and six genes and ESTs originally believed to map to HSA17 were selected for this study. The genes/ESTs were TATA box binding protein-associated factor (TAF2N/RBP56), alpha -2-plasmin inhibitor (SERPINF2/PLI), H3 histone family 3B (H3F3B), aminopeptidase puromycin sensitive (NPEPPS), an expressed sequence tag (ESTMI015) and P311 protein (P311). The SCHP analysis mapped five genes/ESTs (TAF2N, H3F3B, SERPINF2, NPEPPS and ESTMI015) to SSC12q11-q15 and SSC12p11-p15 with 100% concordance, and assigned P311 to SSC2 (1/2q24)-q29 with 100% concordance. Radiation hybrid analysis of all six genes confirmed the SCHP mapping results, with average retention frequency of 25%. Recent human sequence data demonstrated that P311 is actually located on HSA5q. As HSA5q and SSC2q show conserved syntenic regions predicted from bi-directional painting, our P311 mapping data is consistent with these results. An expanded comparative SSC 12 RH map integrating the five new type I markers and 23 previously mapped loci was established using a LOD score threshold of 4.8. The gene order of the five genes/ESTs on the SSC12 framework RH map (H3F3B-ESTMI015-NPEPPS-TAF2N-SERPINF2) is identical to the HSA17 GB4 map but with inversion of the map as conventionally drawn. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a Iowa State University PROMAG Award, and by the Iowa State University Agricultural and Home Economics Experiment Station, project number 3600 |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10635]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_细胞与分子进化开放实验室 |
| 作者单位 | 1.Department of Animal Science, Iowa State University, Ames, IA 50011, USA 2.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, CAS, Kunming 650223, China 3.INRA, Laboratoire de Genetique Cellulaire, 31326 Castanet-Tolosan, France 4.Department of Animal Science, University of Missouri-Columbia, Columbia, MO 65211, USA |
| 推荐引用方式 GB/T 7714 | Shi XW,Fitzsimmons CJ,Green JA,et al. Radiation hybrid comparative mapping between human chromosome 17 and porcine chromosome 12 demonstrates conservation of gene order[J]. ANIMAL GENETICS,2001,32(4):205-209. |
| APA | Shi XW.,Fitzsimmons CJ.,Green JA.,Tuggle CK[*].,Genet C.,...&Whitworth K.(2001).Radiation hybrid comparative mapping between human chromosome 17 and porcine chromosome 12 demonstrates conservation of gene order.ANIMAL GENETICS,32(4),205-209. |
| MLA | Shi XW,et al."Radiation hybrid comparative mapping between human chromosome 17 and porcine chromosome 12 demonstrates conservation of gene order".ANIMAL GENETICS 32.4(2001):205-209. |
入库方式： OAI收割
来源：昆明动物研究所
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