四川和云南汉族长寿老人长寿机理研究
文献类型：学位论文
| 作者 | 龚远英 |
| 学位类别 | 博士 |
| 答辩日期 | 2008-03 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 张亚平 |
| 关键词 | 健康长寿 血糖 TC TG HDL-C LDL-C IL6 IL10 TNFα SNPs |
| 其他题名 | Longevity Mechanism on Han People in Sichuan and Yunnan |
| 学位专业 | 动物学 |
| 中文摘要 | 随着社会的发展，人均寿命的延长，人口老龄化已经成为我国不可回避的社会问题。老年人在医疗卫生保健方面的花费远远超过普通人群。健康长寿老人能避免或者延缓年龄相关疾病的发生，从而减少在卫生保健方面的花费；另外，长寿老人的子女在年龄相关疾病方面的患病率也显著低于年龄相匹配的对照组。因此，开展健康长寿方面的研究，一方面可以提高老年人的生活质量，另一方面可以改善由于人口老龄化而引起的社会问题。 我国长寿老人大多零散分布在乡区，并且由于长寿老人的身体条件等原因，给完整的收集相关数据带来困难。国内关于长寿的研究主要集中在城镇，而且由于人群、地域、生活方式等差异我们也不能完全借鉴国外的研究成果。因此,我们对四川和云南汉族健康长寿老人进行横断面研究,并对其长寿机理进行探索。从亲缘关系上我们把样品分为：长寿组、血缘组和无血缘组三个组；从年龄上分为：≤59、60-89、90-94和≥95四个组。在实验中，我们测定了常规体检指标，常见促炎细胞因子和抗炎细胞因子水平以及其调控区的单核苷酸多态性。 一般认为随着年龄增加血糖水平升高，并且高水平的 HDL-C和高比率的HDL-C/TC以及低TC,TG和LDL-C水平有利于长寿。都江堰长寿老人血糖水平偏低，显著低于血缘组和无血缘组以及其他年龄组；调整年龄后，都江堰≥95岁年龄组的血糖水平显著高于90-94年龄组；血糖水平在性别间不存在显著差异。都江堰长寿老人LDL-C水平在90-94和≥95年龄组显著低于其他年龄组，≥95年龄组显著低于90-94年龄组；调整性别后，90-94年龄组女性的LDL-C水平显著高于男性水平，其他年龄组在性别间差异不显著；女性≥95年龄组显著低于90-94年龄组，但在男性中差异不显著。TG和TC水平在都江堰和红塔两地各组中的变化趋势都存在着差异，在性别间比较发现两地各年龄组变化趋势都一致，仅在90-94年龄组女性都显著高于男性。HDL-C水平和HDL-C/TC在都江堰和红塔两地各组中变化趋势不一致，调整性别后HDL-C/TC在90-94年龄组两地女性的比率都显著大于男性。通常IL6、IL10和TNFα水平随着年龄增加而升高。云南长寿老人的IL6水平显著高于血缘组和无血缘组，性别之间的差异不显著。IL10水平长寿组显著高于无血缘组，TNFα水平在各组间差异不显著，由于这两种细胞因子在绝大多数个体中呈本底水平表达，很难分析年龄与它们水平的相关性。在这三种细胞因子调控区的常见SNPs位点上，许多研究表明il10-1082和il6-174位点和长寿存在着一定的相关性，tnfα-308位点与长寿不存在相关性。在我们收集的样品中il6-174位点不存在多态性。男性个体的il10-592A和-819T的等位基因频率在长寿组显著高于无血缘组，在-1082位点与长寿不存在相关性。tnfα-238A和-308A等位基因频率在长寿组中显著高于无血缘组，在各组单倍型间的差异显著，G-G单倍型在长寿组中呈下降趋势；调整性别后，两位点的等位基因频率在各组间的差异不显著，但单倍型在各组间差异显著，变化趋势同调整性别前一致。 总之，四川都江堰长寿人群的血糖水平和LDL-C水平比较低，都江堰和云南红塔两地长寿老人的血脂水平存在着地域差异。与前人的研究结果比较,汉族人群的IL6、IL10和TNFα水平以及常见的启动区SNPs位点可能存在人种差异。 |
| 英文摘要 | China is suffering from population aging with growing economy. Aging population costs more on medical and health care, while the health longevity population reduces the cost due to sparing age-related diseases. Their offspring also have low prevalence of age-related diseases. Hence, study on health longevity will improve the quality of life in aging population and also reduce social burdens. In China, it is difficult to collect perfect data about nonagenarians and centenarians because their distributions. Health statuses of long-lived people also bring the trouble to collect data. Until now, many studies are conducted in metropolises. In addition, the results in other populations shouldn´t accept without correction owning to the difference in life-style, area and race. Therefore, cross-sectional study of health status on longevity populations in both Sichuan and Yunnan provinces was conducted. Levels and common promoter SNPs of IL6, IL10 and TNFα were also done. All samples were divided into three groups by relationship: longevity group, sibship group and nonsibship group, and four groups by age: ≤59, 60-80, 90-94 and ≥95 years old. Generally, Glu increases with increasing age. High HDL-C, high ratio of HDL-C/TC，and lower levels of LDL-C, TC, Glu and TG take advantageous for extreme life. Glu and blood lipids in longevity group were lower than other groups. 90-94 group was lower than ≥95. No significant difference was found between genders within each age group. LDL-C in longevity was lower than other groups including age-groups in Dujiangyan area. LDL-C in ≥95 group was lower than 90-94. The female LDL-C in 90-94 was higher than ≥95, but no difference in male. Within 90-94, the level in female was higher than in male. The trends of change of TC and TG levels in Dujiangyan were inconsistent with these in Hongta area. Female TC and TG in 90-94 were higher than male in 90-94. HDL-C and HDL-C/TC were of inconsistency in the trend of changes between Dujiangyan and Hongta. After adjusting gender, female HDL-C/TC was greater than male within 90-94 group. Usually, IL6, IL10 and TNFα increased with growing age. Our data showed IL6, IL10 and TNFα were at basal level. IL6 level was higher than sibship and nonsibship group and no difference between genders within each group. No difference in TNFα was also found between groups. IL10 in longevity group was higher than nonsibship group. In many studies, il10-1082 and il6-174 loci have association with longevity and no association at tnfα-308 with longevity. No polymorphism was found at il6-174 from our Han samples. Allele frequency of il10-592A and -819T in male longevity group were higher than in male nonsibship group. The frequency of -238A and -308A was higher in longevity group than in nonsibship group, and the haplotype G-G lower. There were no differences after adjusting genders in -238 and -308 loci except haplotypes. Summarily, Glu and LDL-C were low in dujiangyan longevity group. Regional difference was observed in Glu and lipid profile. Han people have different loci of common SNPs in promoter region of IL6, IL10 and TNFα between longevity group and nonsibship group compared with other populations. |
| 语种 | 中文 |
| 公开日期 | 2010-10-14 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6099]  |
| 专题 | 昆明动物研究所_分子进化基因组学 |
| 推荐引用方式 GB/T 7714 | 龚远英. 四川和云南汉族长寿老人长寿机理研究[D]. 北京. 中国科学院研究生院. 2008. |
入库方式： OAI收割
来源：昆明动物研究所
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