Recovery of behavioral symptoms in hemi-parkinsonian rhesus monkeys through combined gene and stem cell therapy
文献类型：期刊论文
| 作者 | Zhou Y1; Sun MS1; Li HJ1; Yan M1; He ZL1; Wang WJ2; Wang WP3; Lu SY[*]1 |
| 刊名 | CYTOTHERAPY
 |
| 出版日期 | 2013 |
| 卷号 | 15期号:4页码:467-480 |
| 关键词 | Rhesus adipose stem cells (rASCs) TH differentiation LMX1A Neurturin neuron Parkinson disease |
| 通讯作者 | lihj6912@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Background aims. The use of adipose mesenchymal stromal cells (ASCs) in cellular and genic therapy has attracted considerable attention as a possible treatment for neurodegenerative disorders, including Parkinson disease. However, the effects of gene therapy combined with intracerebral cell transplantation have not been well defined. Recent studies have demonstrated the respective roles of LIM homeobox transcription factor 1, alpha (LMX1A) and Neurturin (NTN) in the commitment of embryonic stem cells (ESCs) to a midbrain dopaminergic neuronal fate and the commitment of mesenchymal stromal cells to cells supporting the nutrition and protection of neurons. Methods. We investigated a novel in vitro neuronal differentiation strategy with the use of LMX1A and Neurturin. We were able to elicit a neural phenotype regarding cell morphology, specific gene/protein expression and physiological function. Neuronal-primed ASCs derived from rhesus monkey (rASCs) combined with adenovirus containing NTN and tyrosine hydroxylase (TH) (Ad-NTN-TH) were implanted into the striatum and substantia nigra of methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-lesioned hemi-parkinsonian rhesus monkeys. Monkeys were monitored with the use of behavioral tests and health measures until the fourth month after implantation. Results. The differentiated cells transcribed and expressed a variety of dopaminergic neuron-specific genes involved in the SHH/LMX1A pathway. Single-photon emission computed tomography analysis and postmortem analysis revealed that the grafting of rASCs combined with Ad-NTN-TH had neuroprotective effects compared with Ad-NTN-TH or rASCs alone. Behavioral measures demonstrated autograft survival and symptom amelioration. Conclusions. These findings may lead to cellular sources for autologous transplantation of Parkinson disease. Combined transplantation of Ad-NTN-TH and induced rASCs expressing LMX1A and NTN may be a better therapy candidate for the treatment of Parkinson disease. |
| 收录类别 | SCI |
| 资助信息 | This research was financially supported by the Re- search Fund for Doctor Innovation of Beijing Union Medical College; Science and Technology Pro- ject of Yunnan Province (2012AE001, 2007C0012Z); National Natural Science Foundation of China (30971003). |
| 语种 | 英语 |
| WOS记录号 | WOS:000316535400008 |
| 公开日期 | 2014-10-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8090]  |
| 专题 | 昆明动物研究所_分子免疫生物学 |
| 作者单位 | 1.Institute of Medical Biology, Peking Union Medical College, Chinese Academy of Medical Science, Kunming, China 2.Kunming Institute of Zoology, CAS, Kunming, China 3.First People’s Hospital of Yunnan Province, Kunming, China |
| 推荐引用方式 GB/T 7714 | Zhou Y,Sun MS,Li HJ,et al. Recovery of behavioral symptoms in hemi-parkinsonian rhesus monkeys through combined gene and stem cell therapy[J]. CYTOTHERAPY,2013,15(4):467-480. |
| APA | Zhou Y.,Sun MS.,Li HJ.,Yan M.,He ZL.,...&Lu SY[*].(2013).Recovery of behavioral symptoms in hemi-parkinsonian rhesus monkeys through combined gene and stem cell therapy.CYTOTHERAPY,15(4),467-480. |
| MLA | Zhou Y,et al."Recovery of behavioral symptoms in hemi-parkinsonian rhesus monkeys through combined gene and stem cell therapy".CYTOTHERAPY 15.4(2013):467-480. |
入库方式： OAI收割
来源：昆明动物研究所
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