Molecular Identification and Expression Analysis of the Interferon Beta Gene in Sheep (Ovis aries)
文献类型：期刊论文
| 作者 | Gao HF1,3,4; Li ML1,3; Xu WW1,3; Yang SB4; Xin AG4; Li HC4; Zhang HT[*]1,2 |
| 刊名 | JOURNAL OF ANIMAL AND VETERINARY ADVANCES
 |
| 出版日期 | 2013 |
| 卷号 | 12期号:3页码:412-418 |
| 关键词 | Sheep interferon beta tissue distribution amino acid chelate |
| 合作状况 | 其它 |
| 英文摘要 | Interferon beta (IFN-beta) belongs to the group of inflammatory cytokines that are expressed in eukaryotic cells as an early response to viral infection Sheep as an important livestock m the world, however, little is known about sheep Interferon beta (sIFN-beta) and its role m immune responses In this study, researchers amplified the cDNA of sIFN-beta from sheep spleen by RT-PCR using primers according to the cattle IFN-beta The ORF of sIFN-beta covers 561 bp encoding 186 amino acids Sequence prediction shows that the amino acid of sIFN-beta possess 22 aa signal peptide domain and two putative N-glycosylationlation sites qPCR analysis indicated that sIFN-beta mRNA was predominantly expressed in lymphoid node The 36 kDa recombinant sIFN-beta protein was efficiently expressed and purified using metal chelate affinity chromatography (Ni-NTA) then confirmed by SDS-PAGE and Western blotting Tins study provides a deeper understanding of sIFN-beta molecule structure and also contributes to further studies |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324529400020 |
| 公开日期 | 2015-01-05 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8211]  |
| 专题 | 昆明动物研究所_分子免疫生物学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming, Peoples R China 2.Chongqing Acad Sci & Technol, Chongqing Ctr Biomed Res & Equipment Dev, Chongqing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Yunnan Anim Sci & Vet Inst, Kunming 650224, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao HF,Li ML,Xu WW,et al. Molecular Identification and Expression Analysis of the Interferon Beta Gene in Sheep (Ovis aries)[J]. JOURNAL OF ANIMAL AND VETERINARY ADVANCES,2013,12(3):412-418. |
| APA | Gao HF.,Li ML.,Xu WW.,Yang SB.,Xin AG.,...&Zhang HT[*].(2013).Molecular Identification and Expression Analysis of the Interferon Beta Gene in Sheep (Ovis aries).JOURNAL OF ANIMAL AND VETERINARY ADVANCES,12(3),412-418. |
| MLA | Gao HF,et al."Molecular Identification and Expression Analysis of the Interferon Beta Gene in Sheep (Ovis aries)".JOURNAL OF ANIMAL AND VETERINARY ADVANCES 12.3(2013):412-418. |
入库方式： OAI收割
来源：昆明动物研究所
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