Construction and characterization of Lactobacillus pentosus expressing the D antigenic site of the spike protein of Transmissible gastroenteritis virus
文献类型：期刊论文
| 作者 | Liu DQ1,2; Qiao XY1; Li YJ[*]1; Ge JW1; Tang LJ1; Jiang YP1 |
| 刊名 | CANADIAN JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 57期号:5页码:392-397 |
| 关键词 | Lactobacillus pentosus Transmissible gastroenteritis virus S protein D site |
| 通讯作者 | yijingli@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | This study explored the feasibility of Lactobacillus pentosus as a live vehicle to deliver and express antigen. First of all, L. pentosus transformed by electroporation with the plasmids pg611-6D (anchored) and pg612-6D (secretory) based on the xylose operon generated the recombinant strains rLppg611-6D and rLppg612-6D, respectively, expressing the D antigenic site of the spike (S) protein of Transmissible gastroenteritis virus (TGEV), for intragastric administration in mice. Secondly, we collected serum, fecal, nasal, ophthalmic, and vaginal samples from pre-immune mice and after the first immunization (on days 7, 14, 21, 28, 35, and 42) that were used to analyze the levels of immunoglobulins G and A against TGEV by using ELISA. In addition, a plaque reduction assay was performed using sera from groups pg611, pg612-6D, pg11-6D, and phosphate-buffered saline (blank control) to analyze TGEV-neutralizing antibody activity in vitro. A statistically significant difference in serum tests between groups demonstrated that rLppg612-6D induced better immunogenicity than rLppg611-6D, making rLppg612-6D the better candidate for oral vaccine. Taken together, L. pentosus possessed the potential to become a novel vector for mucosal vaccine in the future. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Cultivation Fund of the Key Scientific and Technical Innovation Project, Education Ministry of China (No. 706019). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10426]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, the People’s Republic of China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, the People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Liu DQ,Qiao XY,Li YJ[*],et al. Construction and characterization of Lactobacillus pentosus expressing the D antigenic site of the spike protein of Transmissible gastroenteritis virus[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2011,57(5):392-397. |
| APA | Liu DQ,Qiao XY,Li YJ[*],Ge JW,Tang LJ,&Jiang YP.(2011).Construction and characterization of Lactobacillus pentosus expressing the D antigenic site of the spike protein of Transmissible gastroenteritis virus.CANADIAN JOURNAL OF MICROBIOLOGY,57(5),392-397. |
| MLA | Liu DQ,et al."Construction and characterization of Lactobacillus pentosus expressing the D antigenic site of the spike protein of Transmissible gastroenteritis virus".CANADIAN JOURNAL OF MICROBIOLOGY 57.5(2011):392-397. |
入库方式： OAI收割
来源：昆明动物研究所
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