Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells
文献类型：期刊论文
| 作者 | Huang H1; Chan H1; Wang YY2,3; Ouyang DY2,3; Zheng YT2; Tam SC[*]1 |
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2006 |
| 卷号 | 79期号:13页码:1287-1292 |
| 关键词 | trichosanthin HSV-1 MAPK Bcl-2 antiviral activity |
| ISSN号 | 0024-3205 |
| 通讯作者 | mtam@cuhk.edu.hk |
| 合作状况 | 其它 |
| 英文摘要 | Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) effective against HIV-1 and HSV-1 replication. The mechanism of its antiviral activity is not clear. Many believe that it is related to ribosome inactivation. Some RIPs and viral infection affect the phosphorylation of MAPK and Bcl-2 and these proteins may be the common element linking RIP and viral infection. This study investigated the effect of HSV-1 infection on p38 MAPK and Bcl-2 as well as possible interference by TCS. Results showed that HSV-1 infection induced an elevation of phosphorylated p38 and Bcl-2 in Vero cells, which could be partially blocked by TCS. At the same time, both viral replication and host cells viability were lowered. Viral replication, Vero cell viability, p38 MAPK and Bcl-2 were further reduced with the addition of a p38 MAPK inhibitor (SB203580). This suggested that TCS may interfere with MAPK and Bcl-2 signals generated by infection leading to inhibition of viral replication. In summary, our results demonstrated that HSV-1 infection in Vero cells induced an elevation of p38 MAPK and Bcl-2. TCS suppressed this rise and reduced viral replication. The MAPK family may play a role in the antiviral mechanism of TCS. |
| 收录类别 | SCI |
| 资助信息 | The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). |
| 原文出处 | 2006791287.pdf |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4557]  |
| 专题 | 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Department of Physiology, The Chinese University of Hong Kong, Hong Kong SAR, China 2.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Huang H,Chan H,Wang YY,et al. Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells[J]. LIFE SCIENCES,2006,79(13):1287-1292. |
| APA | Huang H,Chan H,Wang YY,Ouyang DY,Zheng YT,&Tam SC[*].(2006).Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells.LIFE SCIENCES,79(13),1287-1292. |
| MLA | Huang H,et al."Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells".LIFE SCIENCES 79.13(2006):1287-1292. |
入库方式： OAI收割
来源：昆明动物研究所
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