An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin
文献类型：期刊论文
| 作者 | Ouyang DY1,3; Chan H2; Wang YY1,3; Huang H2; Tam SC[*]2; Zheng YT[*]1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2006 |
| 卷号 | 339期号:1页码:25-29 |
| 关键词 | Trichosanthin Ribosome inactivating proteins HIV c-Jun N-terminal kinases MAPK Anti-HIV activity CEP-11004 |
| ISSN号 | 0006-291X |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Trichosanthin (TCS) is a type I ribosome-inactivating protein possessing multiple biological and pharmacological activities. One of its major actions is inhibition of human immunodeficiency virus (HIV) replication. The mechanism is still not clear. It is generally believed that this action is mediated via ribosome inactivation. Recently, we found that some TCS mutants with full ribosome inactivating activity were devoid of anti-HIV-1 effect. This suggested that there might be other mechanisms contributing to the anti-HIV-1 action. This study showed that a commonly used c-Jun N-terminal kinases inhibitor (CEP-11004) could counteract the antiviral action of TCS in C8166 cells. CEP-11004 alone had no effect on HIV-1 replication and TCS alone significantly inhibited this process. When CEP-11004 was used together with TCS, the antiviral action of TCS was much reduced. Two methods were used to assess viral replication. (1) By measuring the HIV-1 reverse transcriptase, TCS on the average reduced viral replication to 52+/-4%. With CEP-11004 pretreatment, TCS appeared to lose the HIV-1 inhibitory activity with viral replication stood at 101+/-7%. (2) By measuring HIV-1 p24, TCS reduced viral replication to 68+/-4%. With CEP-11004 pretreatment, TCS again seemed to lose its anti-HIV-1 activity with HIV-1 replication rose back to 101+/-4%. Both indexes indicated that CEP-11004 counteracted the antiviral action of TCS. Phosphorylation of JNK on the other hand was only slightly elevated by 1.5-fold by TCS and CEP-11004 inhibited this elevation. These results suggested that the anti-HIV-1 effect of TCS may be related to the MAPK signal process downstream from the point of CEP inhibition. |
| 收录类别 | SCI |
| 资助信息 | The work was supported by grants to Dr. Zheng from the Natural Science Foundation of Chi- na (30471605), the Natural Science Foundation of Yunnan (2003C0001R), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Innovation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Pro- gram (2003AA219142). |
| 原文出处 | 200633925.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4575]  |
| 专题 | 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Department of Physiology, The Chinese University of Hong Kong, Hong Kong SAR, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Ouyang DY,Chan H,Wang YY,et al. An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,339(1):25-29. |
| APA | Ouyang DY,Chan H,Wang YY,Huang H,Tam SC[*],&Zheng YT[*].(2006).An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,339(1),25-29. |
| MLA | Ouyang DY,et al."An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 339.1(2006):25-29. |
入库方式： OAI收割
来源：昆明动物研究所
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