Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein
文献类型：期刊论文
| 作者 | Liu GJ1; Wang JP1; Xiao JC1,2; Zhao ZW2; Zheng YT[*]1 |
| 刊名 | CELLULAR & MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 4期号:3页码:203-208 |
| 关键词 | monoclonal antibody HIV-1 p24 epitope ELISA |
| ISSN号 | 1672-7681 |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 英文摘要 | HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies (mAbs) p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1IIIB, HIV-1Ada-M, HIV-174V mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research. |
| 收录类别 | SCI |
| 资助信息 | The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). |
| 原文出处 | 20074203.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4583]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Key Laboratory of Conservation and Utilization for Bio-resources, Yunnan University, Kunming, Yunnan 650091, China |
| 推荐引用方式 GB/T 7714 | Liu GJ,Wang JP,Xiao JC,et al. Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2007,4(3):203-208. |
| APA | Liu GJ,Wang JP,Xiao JC,Zhao ZW,&Zheng YT[*].(2007).Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein.CELLULAR & MOLECULAR IMMUNOLOGY,4(3),203-208. |
| MLA | Liu GJ,et al."Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein".CELLULAR & MOLECULAR IMMUNOLOGY 4.3(2007):203-208. |
入库方式： OAI收割
来源：昆明动物研究所
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