Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy
文献类型：期刊论文
| 作者 | Cao XM; Ben KL[*]; Wang YQ; Wang YM |
| 刊名 | ANIMAL REPRODUCTION SCIENCE
 |
| 出版日期 | 1997 |
| 卷号 | 49期号:2-3页码:225-234 |
| 关键词 | acrosome reaction initiator TRITC-PSA Hoechst 33258 ionophore GABA spontaneous acrosome reaction tree shrew sperm |
| ISSN号 | 0378-4320 |
| 通讯作者 | immkiz@public.km.yn.cn |
| 英文摘要 | A number of acrosome reaction (AR) initiators have been found to be effective in inducing AR of human, laboratory and domestic animal sperm. Using an improved simple fluorescence microscopy, effects of gamma-aminobutyric acid (GABA), progesterone and ionophore A23187 on sperm AR of tree shrew, a useful animal model in biomedical research, have been investigated. Spontaneous AR in 4.92-7.53% of viable sperm was observed. Complete AR in 10.31-18.25% of viable tree shrew sperm was obviously induced by 5 microM and 10 microM calcium ionophore A23187, 1 mM GABA, and 5 microM progesterone, and there were no significant differences between their abilities to initiate complete AR. No significant differences of AR percentages between 1- and 2-h treatments with A23187, progesterone and/or GABA were observed. These results suggested that the responses of tree shrew sperm to these AR initiators are similar to that of human and other mammalian sperm. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). |
| 原文出处 | 199749225.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5121]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Peoples Republic of China |
| 推荐引用方式 GB/T 7714 | Cao XM,Ben KL[*],Wang YQ,et al. Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy[J]. ANIMAL REPRODUCTION SCIENCE,1997,49(2-3):225-234. |
| APA | Cao XM,Ben KL[*],Wang YQ,&Wang YM.(1997).Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy.ANIMAL REPRODUCTION SCIENCE,49(2-3),225-234. |
| MLA | Cao XM,et al."Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy".ANIMAL REPRODUCTION SCIENCE 49.2-3(1997):225-234. |
入库方式： OAI收割
来源：昆明动物研究所
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