An improved microtiter assay for evaluating anti-HIV-I neutralizing antibodies from sera or plasma
文献类型：期刊论文
| 作者 | Zhang CY1,2; Chen YY1; Ben KL[*]1,3 |
| 刊名 | BMC INFECTIOUS DISEASES
 |
| 出版日期 | 2003 |
| 卷号 | 3期号:X页码:e30 |
| ISSN号 | 1471-2334 |
| 通讯作者 | kben@vip.sina.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: The anti-HIV-1 neutralizing antibody assay is widely used in AIDS vaccine research and other experimental and clinical studies. The vital dye staining method applied in the detection of anti-HIV-1 neutralizing antibody has been used in many laboratories. However, the unknown factor(s) in sera or plasma affected cell growth and caused protection when the tested sera or plasma was continuously maintained in cell culture. In addition, the poor solubility of neutral red in medium (such as RPMI-1640) also limited the use of this assay. Methods: In this study, human T cell line C8166 was used as host cells, and 3-(4,5-Dimethyl-2-thiazolyl)- 2,5-diphenyl-2H-tetrazolium bromide (MTT) instead of neutral red was used as vital dye. In order to avoid the effect of the unknown factor( s), the tested sera or plasma was removed by a washout procedure after initial 3 - 6 h culture in the assay. Result: This new assay eliminated the effect of the tested sera or plasma on cell growth, improved the reliability of detection of anti-HIV-1 neutralizing antibody, and showed excellent agreement with the p24 antigen method. Conclusion: The results suggest that the improved assay is relatively simple, highly duplicable, cost-effective, and well reliable for evaluating anti-HIV-1 neutralizing antibodies from sera or plasma. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the State 863-Program (2001AA215181). |
| 原文出处 | 2003330.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5259]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Laboratory for Molecular and Cell Immunology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.The School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu 212001, China 3.Kunming Chinawave Biotechnology Company Ltd., Kunming, Yunnan 650106, China |
| 推荐引用方式 GB/T 7714 | Zhang CY,Chen YY,Ben KL[*]. An improved microtiter assay for evaluating anti-HIV-I neutralizing antibodies from sera or plasma[J]. BMC INFECTIOUS DISEASES,2003,3(X):e30. |
| APA | Zhang CY,Chen YY,&Ben KL[*].(2003).An improved microtiter assay for evaluating anti-HIV-I neutralizing antibodies from sera or plasma.BMC INFECTIOUS DISEASES,3(X),e30. |
| MLA | Zhang CY,et al."An improved microtiter assay for evaluating anti-HIV-I neutralizing antibodies from sera or plasma".BMC INFECTIOUS DISEASES 3.X(2003):e30. |
入库方式： OAI收割
来源：昆明动物研究所
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