Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)
文献类型：期刊论文
| 作者 | Ng YM1; Yang YH1; Sze KH2; Zhang X3,4; Zheng YT3; Shaw PC[*]1 |
| 刊名 | JOURNAL OF STRUCTURAL BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 174期号:1页码:164-172 |
| 关键词 | Luffin P1 Ribosome-inactivating peptide Nuclear magnetic resonance Anti-HIV-1 |
| 通讯作者 | pcshaw@cuhk.edu.hk |
| 合作状况 | 其它 |
| 英文摘要 | Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif |
| 收录类别 | SCI |
| 资助信息 | The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). |
| 语种 | 英语 |
| 公开日期 | 2011-04-25 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6603]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.School of Life Sciences and Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China 2.Chemistry Department and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China 3.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Ng YM,Yang YH,Sze KH,et al. Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)[J]. JOURNAL OF STRUCTURAL BIOLOGY,2011,174(1):164-172. |
| APA | Ng YM,Yang YH,Sze KH,Zhang X,Zheng YT,&Shaw PC[*].(2011).Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica).JOURNAL OF STRUCTURAL BIOLOGY,174(1),164-172. |
| MLA | Ng YM,et al."Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)".JOURNAL OF STRUCTURAL BIOLOGY 174.1(2011):164-172. |
入库方式： OAI收割
来源：昆明动物研究所
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