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  5. 分子免疫药理学
Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside

文献类型：期刊论文

作者Chen LY1; Li ZX1; Tang YH1; Cui XL3; Luo RH2; Guo SS3; Zheng YT[*]2; Huang CG[*]1
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2011
卷号56期号:2页码:382-389
关键词Calycosin-7-O-beta-D-glucopyranoside Metabolites Antiviral activity
通讯作者zhengyt@mail.kiz.ac.cn ; cghsimm@126.com
合作状况其它
英文摘要In vivo and in vitro metabolites of calycosin-7-O-beta-D-glucopyranoside in rats were identified using a specific and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS(n)) method. The parent compound and twelve metabolites were found in rat urine after oral administration of calycosin-7-O-beta-D-glucopyranoside. The parent compound and six metabolites were detected in rat plasma. In heart, liver, spleen, lung and kidney samples, respectively, six, eight, seven, nine and nine metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat intestinal flora incubation mixture and feces, which demonstrated cleavage of the glycosidic bond of the parent compound in intestines. The main phase I metabolic pathways of calycosin-7-O-beta-D-glucopyranoside in rats were deglycosylation, dehydroxylation and demethylation reactions; phase II metabolism included sulfation, methylation, glucuronidation and glycosylation (probably). Furthermore, two metabolites commonly found in rat urine, plasma and tissues were isolated from feces and characterized by NMR. The antiviral activities of the metabolite calycosin against coxsackie virus B(3) (CVB(3)) and human immunodeficiency virus (HIV) were remarkably stronger than those of calycosin-7-O-beta-D-glucopyranoside.
收录类别SCI
资助信息This work was supported by the National Science & Technol- ogy Major Project “Key New Drug Creation and Manufacturing Program”, China (Grant no. 2009ZX09301-001; 2009ZX09308-005; 2009ZX09501-030; 2009ZX09103-334; 2009ZX09301-005-007; 2009ZX09501-029).
语种英语
公开日期2011-08-09
源URL[http://159.226.149.42:8088/handle/353002/6631]  
专题昆明动物研究所_分子免疫药理学
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Pudong, 201203 Shanghai, China
2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
3.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
推荐引用方式
GB/T 7714		 Chen LY,Li ZX,Tang YH,et al. Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,56(2):382-389.
APA Chen LY.,Li ZX.,Tang YH.,Cui XL.,Luo RH.,...&Huang CG[*].(2011).Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,56(2),382-389.
MLA Chen LY,et al."Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 56.2(2011):382-389.

入库方式： OAI收割

来源：昆明动物研究所

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