Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis
文献类型：期刊论文
| 作者 | Zhang X1,2,3; Yang LM1; Liu GM4; Liu YJ1,3; Zheng CB5; Lv YJ4; Li HZ4; Zheng YT[*]1 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2012 |
| 卷号 | 17期号:6页码:6916-6929 |
| 关键词 | Pinus yunnanensis pine cone anti-HIV activity reverse transcriptase fusion |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 英文摘要 | The anti-HIV activities of a pine cone extract (YNS-PY-F) from Pinus yunnanensis have been evaluated, and its mechanisms of action were also explored. The pine cone extract, YNS-PY-F, potently inhibited HIV-1(IIIB), HIV-1(RF), HIV-1(A17), HIV-1(AO18) and HIV-2(ROD) and induced cytopathic effect in C8166 cells with EC50 values of 0.96 mu g/mL, 1.53 mu g/mL, 0.88 mu g/mL, 7.20 mu g/mL and 6.17 mu g/mL, respectively. The quantification of a p24 production assay showed that YNS-PY-F significantly inhibited the acute replication of HIV-1(IIIB), HIV-1(RF), HIV-1(A17) and HIV-1(AO18) in C8166 cells. An MTT assay showed that YNS-PY-F also significantly inhibited the HIV-1(IIIB) induced cytolysis in MT-4 cells with an EC50 value of 2.22 mu g/mL. The mechanism assays showed that YNS-PY-F had potent inhibitory effects on the fusion between infected cells and uninfected cells, and the activity of HIV-1 reverse transcriptase, with EC50 values of 7.60 mu g/mL and 4.60 mu g/mL, respectively. Overall, these data suggest that the pine cone extract from Pinus yunnanensis has potent inhibitory activities against HIV-1(IIIB), HIV-1(RF), RT inhibitor-resistant strains HIV-1(A17) and HIV-1(AO18), and HIV-2(ROD), and its anti-HIV mechanisms include inhibition of HIV entry and inhibition of reverse transcriptase activity. |
| 收录类别 | SCI |
| 资助信息 | This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). |
| 语种 | 英语 |
| WOS记录号 | WOS:000305800400050 |
| 公开日期 | 2012-09-04 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7012]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Science & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.School of Pharmaceutical Science, Kunming Medical University, Kunming 650500, China 3.Graduate School of the Chinese Academy of Science, Beijing 100039, China 4.School of Pharmacy, Dali University, Dali 671000, China; 5.School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China |
| 推荐引用方式 GB/T 7714 | Zhang X,Yang LM,Liu GM,et al. Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis[J]. MOLECULES,2012,17(6):6916-6929. |
| APA | Zhang X.,Yang LM.,Liu GM.,Liu YJ.,Zheng CB.,...&Zheng YT[*].(2012).Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis.MOLECULES,17(6),6916-6929. |
| MLA | Zhang X,et al."Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis".MOLECULES 17.6(2012):6916-6929. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。