The anti-herpetic activity of trichosanthin via the nuclear factor-kappa B and p53 pathways
文献类型：期刊论文
| 作者 | He DX1; Zheng YT2; Tam SC[*]3 |
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2012 |
| 卷号 | 90期号:17-18页码:673-681 |
| 关键词 | Human herpes simplex virus type 1 Nuclear factor-kappa B p53 Trichosanthin |
| 通讯作者 | MTAM@cuhk.edu.hk |
| 合作状况 | 其它 |
| 英文摘要 | Aims: Trichosanthin (TCS) is a type I ribosome-inactivating protein. We have previously shown that TCS induces a more potent apoptosis in infected cells over uninfected cells, but the mechanism underlying it is unclear. In this study, we explored the anti-HSV-1 mechanism of TCS through the nuclear factor-kappa B (NF-kappa B) and p53 pathways in human epithelial carcinoma (HEp-2) cells with wild type p53. Main methods: The western blot, electrophoretic mobility shift assay, chromatin immunoprecipitation assay. enzyme-linked immunosorbent assay and cytokinesis-block micronucleus were applied in this study. Key findings: It was shown that TCS inhibited the HSV-1-induced NF-kappa B activation. Meanwhile, in HSV-1 infected cells, TCS treatment activated significantly more p53 and BAX, with no DNA damage and less S phase arrest compared with uninfected cells. The activation of BAX in infected cells correlated with the cell death signaling of p53. Significance: Taken together, these results suggest that the anti-HSV-1 effect of TCS is related to its suppression of NF-kappa B activation and regulation of p53-dependent cell death in infected cells. |
| 资助信息 | We appreciate the funding support of the School of Biomedical Sciences of the Chinese University of Hong Kong. |
| 语种 | 英语 |
| WOS记录号 | WOS:000304177300006 |
| 公开日期 | 2012-09-13 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7081]  |
| 专题 | 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.The Key laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, PR China 3.School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China |
| 推荐引用方式 GB/T 7714 | He DX,Zheng YT,Tam SC[*]. The anti-herpetic activity of trichosanthin via the nuclear factor-kappa B and p53 pathways[J]. LIFE SCIENCES,2012,90(17-18):673-681. |
| APA | He DX,Zheng YT,&Tam SC[*].(2012).The anti-herpetic activity of trichosanthin via the nuclear factor-kappa B and p53 pathways.LIFE SCIENCES,90(17-18),673-681. |
| MLA | He DX,et al."The anti-herpetic activity of trichosanthin via the nuclear factor-kappa B and p53 pathways".LIFE SCIENCES 90.17-18(2012):673-681. |
入库方式： OAI收割
来源：昆明动物研究所
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