Identification and characterization of a novel splice variant of rhesus macaque MHC IA
文献类型：期刊论文
| 作者 | Dai ZX1,2; Zhang GH1; Zhang XH1,2; Zheng YT[*]1 |
| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 53期号:3页码:206-213 |
| 关键词 | Rhesus macaque Alternative splicing MHC IA Splice variant cis-Interaction Heterodimer |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Major histocompatibility complex class I (MHC I) molecules play a pivotal role in the immune recognition to intracellular pathogens. A number of important splice variants have already been characterized for these molecules in different species, suggesting their important roles in modulation of immune responses. In this study, we have identified and characterized a novel alternatively spliced form of rhesus macaque MHC IA (designated MHC IA-sv2) that lacks exons coding for the alpha 2 and alpha 3 domains. Despite lacking the alpha 2 and alpha 3 domains, MHC IA-sv2 is targeted to the cell surface, as a 23-kDa glycoprotein that is totally susceptible to endoglycosidase-H digestion and is reduced to 18 kDa after deglycosylation with PNGase F. In contrast, the full-length MHC IA reaches the cell surface as a 43-kDa protein of form with complex-type N-glycosylation (endoglycosidase-H resistant). Moreover, we provide evidence here that MHC IA-sv2 can self-associate, forming homodimers, or associate with the fully mature MHC IA molecule, forming a heterodimeric structure in mammalian cells. These data demonstrate that the formation of heterodimers may have some functional implications in the fine tuning of MHC IA-mediated innate and adaptive immune responses |
| 收录类别 | SCI |
| 资助信息 | The work was supported by the National Basic Research Program of China (2012CBA01305), the National Natural Science Founda- tion of China (30800113; 30872317; U0832601; 81172876), the Knowledge Innovation Program of CAS (KSCX2-EW-R-13), the Key Scientific and Technological Program of China (2012ZX10001-006; 2012ZX10001-007). |
| 语种 | 英语 |
| WOS记录号 | WOS:000310822500005 |
| 公开日期 | 2013-04-09 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7318]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China 2.Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China |
| 推荐引用方式 GB/T 7714 | Dai ZX,Zhang GH,Zhang XH,et al. Identification and characterization of a novel splice variant of rhesus macaque MHC IA[J]. MOLECULAR IMMUNOLOGY,2013,53(3):206-213. |
| APA | Dai ZX,Zhang GH,Zhang XH,&Zheng YT[*].(2013).Identification and characterization of a novel splice variant of rhesus macaque MHC IA.MOLECULAR IMMUNOLOGY,53(3),206-213. |
| MLA | Dai ZX,et al."Identification and characterization of a novel splice variant of rhesus macaque MHC IA".MOLECULAR IMMUNOLOGY 53.3(2013):206-213. |
入库方式： OAI收割
来源：昆明动物研究所
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