Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene
文献类型：期刊论文
| 作者 | Zhang XH1,2; Dai ZX1; Zhang GH1; Han JB1; Zheng YT[*]1 |
| 刊名 | GENE
 |
| 出版日期 | 2013 |
| 卷号 | 522期号:2页码:147-155 |
| 关键词 | Tupaia belangeri Tree shrew Allele Splice variant Haplotype |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The major histocompatibility complex (MHC) class I genes play a pivotal role in the adaptive immune response among vertebrates. Accordingly, in numerous mammals the genomic structure and molecular characterization of MHC class I genes have been thoroughly investigated. To date, however, little is known about these genes in tree shrews, despite the increasingly popularity of its usage as an animal model. To address this deficiency, we analyzed the structure and characteristic of the tree shrew MHC class I genes (Tube-MHC I) and performed a comparative gene analysis of the tree shrew and other mammal species. We found that the full-length cDNA sequence of the tree shrew MHC class I is 1074 bp in length. The deduced peptide is composed of 357 amino acids containing a leader peptide, an alpha 1 and alpha 2 domain, an alpha 3 domain, a transmembrane domain and a cytoplasmic domain. Among these peptides, the cysteines, CD8(+) interaction and N-glycosylation sites are all well conserved. Furthermore, the genomic sequence of the tree shrew MHC class I gene was identified to be 3180 bp in length, containing 8 exons and 7 introns. In 21 MHC class I sequences, we conducted an extensive study of nucleotide substitutions. The results indicated that in the peptide binding region (PBR) the rate of non-synonymous substitutions (dN) to synonymous substitutions (dS) was greater than 1, suggesting balancing selection at the PBR. These findings provide valuable contributions in furthering our understanding of the structure, molecular polymorphism, and function of the MHC class I genes in tree shrews, further improving their utility as an animal model in biomedical research |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the 863 Program of China (2012AA021801), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-J-23), the National Basic Research Program of China (2012CBA01305), and the National Natural Science Foundation of China (30872317; 30800113; U0832601). |
| 语种 | 英语 |
| WOS记录号 | WOS:000320147000004 |
| 公开日期 | 2013-07-18 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7573]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.University of Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Zhang XH,Dai ZX,Zhang GH,et al. Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene[J]. GENE,2013,522(2):147-155. |
| APA | Zhang XH,Dai ZX,Zhang GH,Han JB,&Zheng YT[*].(2013).Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene.GENE,522(2),147-155. |
| MLA | Zhang XH,et al."Molecular characterization, balancing selection, and genomic organization of the tree shrew (Tupaia belangeri) MHC class I gene".GENE 522.2(2013):147-155. |
入库方式： OAI收割
来源：昆明动物研究所
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