Replication potentials of HIV-1/HSIV in PBMCs from northern pigtailed macaque (Macaca leonina)
文献类型：期刊论文
| 作者 | Lei AH1,2; Zhang GH1; Tian RR1,2; Zhu JW1,2; Zheng HY1,3; Pang W[*]1; Zheng YT[*]1,4 |
| 刊名 | Zoological Research
 |
| 出版日期 | 2014 |
| 卷号 | 35期号:3页码:186−195 |
| 关键词 | HIV-1 HSIV Replication PBMC Northern pig-tailed macaque (Macaca leonina) |
| 通讯作者 | zhengyt@mail.kiz.ac.cn ; pangw@mail.kiz.ac.cn |
| 英文摘要 | The northern pig-tailed macaque (Macaca leonina) has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus type 1 (HIV-1), which may be due to the absence of a TRIM5 protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M. leonina. The results indicate that these strains are characterized by various but low replication levels, and among which, HIV-1NL4-3 shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of vif-substituted HIV-1 (HSIV) in M. leonina PBMCs. Notably, HSIV-vifmac and stHIV-1SV chimeras, two HIV-1NL4-3-derived viruses encoding the viral infectivity factor (Vif) protein from SIVmac239, replicated robustly in cells from M. leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M. leonina. Therefore, our data demonstrate that M. leonina has the potential to be developed into a promising animal model for human AIDS. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by the National Basic Research Program (2012CBA01305); the National Natural Science Foundation of China (81172876, U0832601, 81273251 and U1202228); the Knowledge Innovation Program of CAS (KSCX2-EW-R-13, Y206A- 71181), and the Key Scientific and Technological Program of China (2012ZX10001-007, 2013ZX10001-002). |
| 语种 | 英语 |
| 公开日期 | 2014-07-04 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7936]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China 3.University of Science and Technology of China, Hefei 230026, China 4.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Lei AH,Zhang GH,Tian RR,et al. Replication potentials of HIV-1/HSIV in PBMCs from northern pigtailed macaque (Macaca leonina)[J]. Zoological Research,2014,35(3):186−195. |
| APA | Lei AH.,Zhang GH.,Tian RR.,Zhu JW.,Zheng HY.,...&Zheng YT[*].(2014).Replication potentials of HIV-1/HSIV in PBMCs from northern pigtailed macaque (Macaca leonina).Zoological Research,35(3),186−195. |
| MLA | Lei AH,et al."Replication potentials of HIV-1/HSIV in PBMCs from northern pigtailed macaque (Macaca leonina)".Zoological Research 35.3(2014):186−195. |
入库方式： OAI收割
来源：昆明动物研究所
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