Characterization of a MAVS ortholog from the Chinese tree shrew (Tupaia belangeri chinensis)
文献类型：期刊论文
| 作者 | Xu L1; Yu DD1; Peng L1,2; Fan Y1,2; Chen JQ3; Zheng YT1,3; Wang C4; Yao YG[*]1,2,3 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 52期号:1页码:58-68 |
| 关键词 | MAVS Tree shrew Antiviral innate immunity Evolutionary conservation |
| 通讯作者 | yaoyg@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Human mitochondrial antiviral signaling protein (hMAVS, also known as IPS-1, VISA, or Cardif) is essential for antiviral innate immunity. The Chinese tree shrew (Tupaia belangeri chinenses), a close relative of primates, is emerging as a potential animal model for investigating viral infection. However, there is a lack of biological knowledge about the antiviral innate immunity of the tree shrew. In this study, we identified and characterized the function of the Chinese tree shrew MAVS gene (tMAVS). The cDNA of tMAVS was 2771 bp in length and encoded a polypeptide of 501 amino acids. Phylogenetic analyses based on the amino acid sequences revealed a closer affinity of tMAVS with those of primates. Quantitative real-time PCR analysis indicated that tMAVS mRNA was constitutively expressed in all seven tissues analyzed in this study. The tMAVS mRNA expression was rapidly and significantly increased after RNA virus infections. Ectopic-expression of tMAVS significantly potentiated the virus-triggered activation of IRF3, NF-kappa B and interferon-beta (IFN-beta), whereas knockdown of tMAVS displayed the opposite effect. Furthermore, tMAVS mutants lacking the caspase activation and recruitment (CARD) domains or the transmembrane (TM) domain were unable to induce IFN-beta. Similar with hMAVS, mitochondrial localization of tMAVS was dependent on its domain. Collectively, this study revealed evolutionary conservation of the MAVS antiviral signaling pathway in the Chinese tree shrew. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by the Na- tional Natural Science Foundation of China (U1402224), the National 863 Project of China (2012AA021801) and grants from the Chinese Academy of Sciences (KSCX2-EW-R-11). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9239]  |
| 专题 | 昆明动物研究所_分子免疫药理学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China 3.Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China |
| 推荐引用方式 GB/T 7714 | Xu L,Yu DD,Peng L,et al. Characterization of a MAVS ortholog from the Chinese tree shrew (Tupaia belangeri chinensis)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(1):58-68. |
| APA | Xu L.,Yu DD.,Peng L.,Fan Y.,Chen JQ.,...&Yao YG[*].(2015).Characterization of a MAVS ortholog from the Chinese tree shrew (Tupaia belangeri chinensis).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(1),58-68. |
| MLA | Xu L,et al."Characterization of a MAVS ortholog from the Chinese tree shrew (Tupaia belangeri chinensis)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.1(2015):58-68. |
入库方式： OAI收割
来源：昆明动物研究所
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