Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity
文献类型：期刊论文
| 作者 | Wang JH1; Nie HL2; Tam SC3; Huang H3; Zheng YT[*]1 |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 531期号:2页码:295-298 |
| 关键词 | trichosanthin ribosome inactivating protein human immunodeficiency virus type 1 antiviral activity |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCSM((120-123)), TCSE160A/E189A, with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCSR122G, which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a grant from The Na- tional Natural Science Foundation of China (39970851) and Natural Science Foundation of Yunnan (1999C0087M). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9678]  |
| 专题 | 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China 2.Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, PR China 3.Department of Physiology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China |
| 推荐引用方式 GB/T 7714 | Wang JH,Nie HL,Tam SC,et al. Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity[J]. FEBS LETTERS,2002,531(2):295-298. |
| APA | Wang JH,Nie HL,Tam SC,Huang H,&Zheng YT[*].(2002).Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity.FEBS LETTERS,531(2),295-298. |
| MLA | Wang JH,et al."Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity".FEBS LETTERS 531.2(2002):295-298. |
入库方式： OAI收割
来源：昆明动物研究所
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