Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronicaly infected T-lymphocytes
文献类型：期刊论文
| 作者 | Zheng YT1; Ben KL[*]1; Jin SW2 |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999 |
| 卷号 | 20期号:3页码:239-243 |
| 关键词 | alpha-momorcharin ribosome-inactivating proteins HIV antigen p24 giant cells T-lymphocytes anti-HIV agents cytotoxicity |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 中文摘要 | 目的：研究α 苦瓜子蛋白（α ＭＭＣ）在ＨＩＶ １急性和慢性感染Ｔ淋巴细胞中的抗ＨＩＶ １活性．方法：以合胞体抑制实验，ＨＩＶ １ｐ２４核心抗原表达水平和ＨＩＶ抗原阳性细胞的百分率检测了α ＭＭＣ的抗ＨＩＶ活性．结果：α ＭＭＣ对ＨＩＶ １ⅢＢ诱导Ｃ８１６６细胞形成合胞体有显著的抑制作用．α ＭＭＣ显著地抑制了ＨＩＶ １急性感染Ｔ细胞中ｐ２４抗原的表达水平和减少了ＨＩＶ抗原阳性细胞的百分率．在上述三种测定中，α ＭＭＣ的ＥＣ５０分别是００１６，００７和０３２ｍｇ·Ｌ－１．但是，α ＭＭＣ不影响慢性感染ＨＩＶ １ⅢＢ／Ｈ９中ｐ２４抗原表达水平．结论：α ＭＭＣ是苦瓜子蛋白中唯一的具有抗ＨＩＶ １活性的成份．α ＭＭＣ抑制急性感染中ＨＩＶ １的复制而对慢性感染Ｔ细胞中的ＨＩＶ １复制无影响 |
| 英文摘要 | AIM: To identify the anti-human immunodeficiency virus type 1 (HIV-1) activities of alpha-momorcharin ( alpha-MMC) from Momordica charantia in acutely and chronically infected lymphocytes. METHODS: The anti-HIV activities of alpha-MMC were examined by 1) the inhibition of syncytia formation induced by HIV-1 III B; 2) reduction of p24 core antigen expression level and decrease in numbers of HIV antigen positive cells in acutely and chronically infected cultures. The cytotoxic effects of alpha-MMC was tested by trypan blue dye exclusion or colorimetric MTT assay. RESULTS: alpha-MMC was found to obviously inhibit HIV-1 III B-inducing C8166 syncytia formation and markedly reduced both expression of p24 core antigen and the numbers of HIV antigen positive cells in acutely but not chronically HTV-1-infected culture. The median effective concentration (EC50) in these assays were 0.016, 0.07, and 0.32 mg.L-1, respectively. CONCLUSION: alpha-MMC is a unique component of momorcharin with anti-HIV activity, and markedly inhibited HIV-1 replication in acutely but not chronically HIV-1-infected T-lymphocytes. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9821]  |
| 专题 | 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng YT,Ben KL[*],Jin SW. Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronicaly infected T-lymphocytes[J]. ACTA PHARMACOLOGICA SINICA,1999,20(3):239-243. |
| APA | Zheng YT,Ben KL[*],&Jin SW.(1999).Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronicaly infected T-lymphocytes.ACTA PHARMACOLOGICA SINICA,20(3),239-243. |
| MLA | Zheng YT,et al."Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronicaly infected T-lymphocytes".ACTA PHARMACOLOGICA SINICA 20.3(1999):239-243. |
入库方式： OAI收割
来源：昆明动物研究所
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