Evaluation of Cynanchum otophyllum glucan sulfate against human immunodeficiency virus and herpes simplex virus as a microbicide agent
文献类型：期刊论文
| 作者 | Tao J1,2; Yang J2,3; Ben KL[*]2; Chen CY4; Cao XM2; Zhao SL5 |
| 刊名 | INDIAN JOURNAL OF PHARMACOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:5页码:536-540 |
| 关键词 | Cynanchum otophyllum Human Immunodeficiency Virus Herpes Simplex Virus microbicide |
| 通讯作者 | kben@vip.sina.com |
| 合作状况 | 其它 |
| 英文摘要 | Objective: The root of Cynanchum otophyllum-also known as Qing Yang Sheng-is a traditional ethnical Chinese medicine. The objective of this study was to evaluate in vitro activities and safety of C. otophyllum glucan sulfate (PS20) against Human Immunodeficiency Virus (HIV) and Herpes Simplex Virus (HSV). Materials and Methods: Anti-HIV activity was detected with syncytial formation assay and quantitative P24 Enzyme-Linked Immunosorbent Assay (ELISA). Anti-HSV activity was detected with plaque reduction assay; cytotoxicity was tested with MTT colorimetric assay; and anti-bacterial activity was tested with microdilution method. Anti-HIV mechanism was investigated with fusion inhibition, time of addition, and pretreatment. Results: The 50% Inhibition Concentration (IC(50)) of PS20 for HIV-1(IIIB), HIV-(Ada-M), HIV-1(Bal), HSV-I, and -II were 0.26 +/- 0.02 mu M, 0.46 +/- 0.02 mu M, 0.90 +/- 0.04 mu M, 3.45 +/- 0.85 mu M, and 0.70 +/- 0.22 mu M, respectively. Selectivity Indices (SI) were 653, 50, 39, 85, and 362, respectively. Studies on anti-HIV mechanism of PS20 showed that the target molecule should be the envelope protein. The 50% Cytotoxicity Concentrations (CC(50)) of PS20 for HeLa and ME-180 cell lines and human foreskin fibroblast cells was more than 70 mu M. The Minimum Inhibitory Concentration (MIC) for vaginal lactobacilli was more than 1000 mu M. Conclusion: PS20 possesses anti-HIV and HSV effect and low cytotoxicity to epithelium cells and vaginal lactobacilli. It may be considered as a potential microbicide agent for further investigation. |
| 语种 | 中文 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10412]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Pharmacology, Kunming University, Yunnan, Republic of China 2.Laboratory of Cell and Molecular Immunology, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Republic of China 3.Department of Chemical Processing of Forest Products, Southwest Forestry University, Yunnan, Republic of China 4.Department of Life Science and Technology, Kunming University of Science and Technology, Yunnan, Republic of China 5.Department of Chinese Materia Medica, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, Republic of Republic of China |
| 推荐引用方式 GB/T 7714 | Tao J,Yang J,Ben KL[*],et al. Evaluation of Cynanchum otophyllum glucan sulfate against human immunodeficiency virus and herpes simplex virus as a microbicide agent[J]. INDIAN JOURNAL OF PHARMACOLOGY,2011,43(5):536-540. |
| APA | Tao J,Yang J,Ben KL[*],Chen CY,Cao XM,&Zhao SL.(2011).Evaluation of Cynanchum otophyllum glucan sulfate against human immunodeficiency virus and herpes simplex virus as a microbicide agent.INDIAN JOURNAL OF PHARMACOLOGY,43(5),536-540. |
| MLA | Tao J,et al."Evaluation of Cynanchum otophyllum glucan sulfate against human immunodeficiency virus and herpes simplex virus as a microbicide agent".INDIAN JOURNAL OF PHARMACOLOGY 43.5(2011):536-540. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。