Allosteric regulation of the calcium-sensing receptor in obese individuals
文献类型：期刊论文
| 作者 | He YH1,2; Perry B3; Bi MX1; Sun H4; Zhao TT5; Li Y[*]1; Sun CH[*]1 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
 |
| 出版日期 | 2013 |
| 卷号 | 32期号:2页码:511-518 |
| 关键词 | calcium-sensing recepor obesity cytokine calcium amino acids |
| 通讯作者 | liying_helen@163.com ; sun2002changhao@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | We have previously reported that the calcium-sensing receptor (CaSR) plays an important role in modulating lipid metabolism under low calcium conditions. The aim of this study was to identify possible regulators of CaSR and the mechanisms of action of CaSR in obese individuals. Subcutaneous fat samples were obtained from 10 obese and 10 non-obese males undergoing elective abdominal surgery. Visceral fat pads were obtained from 12 obese and 12 non-obese male rats. Serum lipid, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) concentrations, as well as the gene and protein expression of CaSR in the white adipose tissue of obese subjects and rats were determined. Serum total calcium, vitamin D, parathyroid hormone (PTH) and amino acid levels in human subjects were measured. Intracellular calcium and cyclic adenosine monophosphate (cAMP) levels in rat adipocytes were measured by laser scanning confocal microscopy and ELISA, respectively. The results revealed that serum triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), TNF-alpha, IL-6 and PTH levels were significantly higher in the obese individuals versus the controls. By contrast, serum vitamin D and amino acid concentrations were lower in the obese individuals versus the controls. In addition, intracellular calcium levels were higher, while cAMP levels were lower in the obese rat adipocytes compared with the control group. However, the gene and protein expression of CaSR in white adipose tissue did not differ between the obese groups and the controls. Thus, these results suggest that CaSR functions not through its expression, but rather through allosteric regulation in obese individuals. |
| 收录类别 | SCI |
| 资助信息 | This study was funded by grants from the National Natural Science Fund of China key project (no. 81130049) and the Program for New Century Excellent Talents in University of China (no. NCET-10-0148). |
| 语种 | 英语 |
| WOS记录号 | WOS:000322370800032 |
| 公开日期 | 2013-08-30 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7620]  |
| 专题 | 昆明动物研究所_分子人类学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.National Key Discipline of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. China 3.Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, Prince Edward Island C1A 4P3, Canada 4.Emergency Department, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China 5.Department of Psychology, University of Toronto, Toronto, Ontario M1C 1A4, Canada |
| 推荐引用方式 GB/T 7714 | He YH,Perry B,Bi MX,et al. Allosteric regulation of the calcium-sensing receptor in obese individuals[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2013,32(2):511-518. |
| APA | He YH.,Perry B.,Bi MX.,Sun H.,Zhao TT.,...&Sun CH[*].(2013).Allosteric regulation of the calcium-sensing receptor in obese individuals.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,32(2),511-518. |
| MLA | He YH,et al."Allosteric regulation of the calcium-sensing receptor in obese individuals".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 32.2(2013):511-518. |
入库方式： OAI收割
来源：昆明动物研究所
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