Radicicol, a heat shock protein 90 inhibitor, inhibits differentiation and adipogenesis in 3T3-L1 preadipocytes
文献类型：期刊论文
| 作者 | He YH1,2,5; Li Y1; Zhang SC2; Perry B2,4; Zhao TT2,3; Wang YW[*]2,4; Sun CH[*]1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2013 |
| 卷号 | 436期号:2页码:169-174 |
| 关键词 | Radicicol Differentiation Heat shock protein Adipocytes |
| 通讯作者 | yanwen.wang@nrc.ca ; sun2002changhao@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Heat shock protein 90 (Hsp90) is involved in various cellular processes, such as cell proliferation, differentiation and apoptosis. As adipocyte differentiation plays a critical role in obesity development, the present study investigated the effect of an Hsp90 inhibitor radicicol on the differentiation of 3T3-L1 preadipocytes and potential mechanisms. The cells were treated with different concentrations of radicicol during the first 8 days of cell differentiation. Adipogenesis, the expression of adipogenic transcriptional factors, differentiation makers and cell cycle were determined. It was found that radicicol dose-dependently decreased intracellular fat accumulation through down-regulating the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT element binding protein alpha (C/EBP alpha), fatty acid synthase (FAS) and fatty acid-binding protein 4 (FABP4). Flow cytometry analysis revealed that radicicol blocked cell cycle at G1-S phase. Radicicol redcued the phosphorylation of Akt while showing no effect on beta-catenin expression. Radicicol decreased the phosphorylation of phosphoinositide-dependent kinase 1 (PDK1). The results suggest that radicicol inhibited 3T3-L1 preadipocyte differentiation through affecting the PDK1/Akt pathway and subsequent inhibition of mitotic clonal expansion and the expression/activity of adipogenic transcriptional factors and their downstream adipogenic proteins. |
| 收录类别 | SCI |
| 资助信息 | The study was supported by funding from the Canadian Insti- tutes of Health Research (CIHR)-China-Canada Joint Health Re- search Initiative (CCI-92219) and the NRC-MOE Research and Post-doctoral Fellowship Program of Chinese Scholarship Council (CSC). |
| 语种 | 英语 |
| WOS记录号 | WOS:000321601300011 |
| 公开日期 | 2013-08-30 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7622]  |
| 专题 | 昆明动物研究所_分子人类学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Harbin 150081, PR China 2.Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, PE, Canada C1A 4P3 3.Department of Psychology, University of Toronto, 1265 Military Trail, Toronto, ON, Canada M1C 1A4 4.Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, Canada C1A 4P3 5.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China |
| 推荐引用方式 GB/T 7714 | He YH,Li Y,Zhang SC,et al. Radicicol, a heat shock protein 90 inhibitor, inhibits differentiation and adipogenesis in 3T3-L1 preadipocytes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,436(2):169-174. |
| APA | He YH.,Li Y.,Zhang SC.,Perry B.,Zhao TT.,...&Sun CH[*].(2013).Radicicol, a heat shock protein 90 inhibitor, inhibits differentiation and adipogenesis in 3T3-L1 preadipocytes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,436(2),169-174. |
| MLA | He YH,et al."Radicicol, a heat shock protein 90 inhibitor, inhibits differentiation and adipogenesis in 3T3-L1 preadipocytes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 436.2(2013):169-174. |
入库方式： OAI收割
来源：昆明动物研究所
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