Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes
文献类型：期刊论文
| 作者 | He YH1,2,3; Li W4; Li Y1; Zhang SC2,5; Wang YW[*]2,5; Sun CH[*]1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:10页码:e110711 |
| 通讯作者 | yanwen.wang@nrc.ca ; sun2002changhao@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Background: Ursolic acid (UA), a triterpenoid compound, is reported to have a glucose-lowering effect. However, the mechanisms are not fully understood. Adipose tissue is one of peripheral tissues that collectively control the circulating glucose levels. Objective: The objective of the present study was to determine the effect and further the mechanism of action of UA in adipocytes. Methods and Results: The 3T3-L1 preadipocytes were induced to differentiate and treated with different concentrations of UA. NBD-fluorescent glucose was used as the tracer to measure glucose uptake and Western blotting used to determine the expression and activity of proteins involved in glucose transport. It was found that 2.5, 5 and 10 mu M of UA promoted glucose uptake in a dose-dependent manner (17%, 29% and 35%, respectively). 10 mM UA-induced glucose uptake with insulin stimulation was completely blocked by the phosphatidylinositol (PI) 3-kinase (PI3K) inhibitor wortmannin (1 mu M), but not by SB203580 (10 mu M), the inhibitor of mitogen-activated protein kinase (MAPK), or compound C (2.5 mM), the inhibitor of AMP-activated kinase (AMPK) inhibitor. Furthmore, the downstream protein activities of the PI3K pathway, phosphoinositide-dependent kinase (PDK) and phosphoinositide-dependent serine/threoninekinase (AKT) were increased by 10 mM of UA in the presence of insulin. Interestingly, the activity of AS160 and protein kinase C (PKC) and the expression of glucose transporter 4 (GLUT4) were stimulated by 10 mM of UA under either the basal or insulin-stimulated status. Moreover, the translocation of GLUT4 from cytoplasm to cell membrane was increased by UA but decreased when the PI3K inhibitor was applied. Conclusions: Our results suggest that UA stimulates glucose uptake in 3T3-L1 adipocytes through the PI3K pathway, providing important information regarding the mechanism of action of UA for its anti-diabetic effect. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by the Internal Research Project of the Third People’s Hospital of Yunnan Province (YJ201202) and the National Natural Science Fund of China (81130049). |
| 语种 | 英语 |
| WOS记录号 | WOS:000343942100088 |
| 公开日期 | 2014-12-05 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8189]  |
| 专题 | 昆明动物研究所_分子人类学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China 2.Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, Prince Edward Island, Canada 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 4.Department of Endocrinology, Third People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China 5.Department of Biomedical Sciences, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada |
| 推荐引用方式 GB/T 7714 | He YH,Li W,Li Y,et al. Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes[J]. PLOS ONE,2014,9(10):e110711. |
| APA | He YH,Li W,Li Y,Zhang SC,Wang YW[*],&Sun CH[*].(2014).Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes.PLOS ONE,9(10),e110711. |
| MLA | He YH,et al."Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes".PLOS ONE 9.10(2014):e110711. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。