中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes

文献类型:期刊论文

作者He YH1,2,3; Li W4; Li Y1; Zhang SC2,5; Wang YW[*]2,5; Sun CH[*]1
刊名PLOS ONE
出版日期2014
卷号9期号:10页码:e110711
通讯作者yanwen.wang@nrc.ca ; sun2002changhao@yahoo.com
合作状况其它
英文摘要Background: Ursolic acid (UA), a triterpenoid compound, is reported to have a glucose-lowering effect. However, the mechanisms are not fully understood. Adipose tissue is one of peripheral tissues that collectively control the circulating glucose levels. 

Objective: The objective of the present study was to determine the effect and further the mechanism of action of UA in adipocytes. 

Methods and Results: The 3T3-L1 preadipocytes were induced to differentiate and treated with different concentrations of UA. NBD-fluorescent glucose was used as the tracer to measure glucose uptake and Western blotting used to determine the expression and activity of proteins involved in glucose transport. It was found that 2.5, 5 and 10 mu M of UA promoted glucose uptake in a dose-dependent manner (17%, 29% and 35%, respectively). 10 mM UA-induced glucose uptake with insulin stimulation was completely blocked by the phosphatidylinositol (PI) 3-kinase (PI3K) inhibitor wortmannin (1 mu M), but not by SB203580 (10 mu M), the inhibitor of mitogen-activated protein kinase (MAPK), or compound C (2.5 mM), the inhibitor of AMP-activated kinase (AMPK) inhibitor. Furthmore, the downstream protein activities of the PI3K pathway, phosphoinositide-dependent kinase (PDK) and phosphoinositide-dependent serine/threoninekinase (AKT) were increased by 10 mM of UA in the presence of insulin. Interestingly, the activity of AS160 and protein kinase C (PKC) and the expression of glucose transporter 4 (GLUT4) were stimulated by 10 mM of UA under either the basal or insulin-stimulated status. Moreover, the translocation of GLUT4 from cytoplasm to cell membrane was increased by UA but decreased when the PI3K inhibitor was applied. 

Conclusions: Our results suggest that UA stimulates glucose uptake in 3T3-L1 adipocytes through the PI3K pathway, providing important information regarding the mechanism of action of UA for its anti-diabetic effect.
收录类别SCI
资助信息This research was supported by the Internal Research Project of the Third People’s Hospital of Yunnan Province (YJ201202) and the National Natural Science Fund of China (81130049).
语种英语
WOS记录号WOS:000343942100088
公开日期2014-12-05
源URL[http://159.226.149.42:8088/handle/152453/8189]  
专题昆明动物研究所_分子人类学
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China
2.Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, Prince Edward Island, Canada
3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China
4.Department of Endocrinology, Third People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China
5.Department of Biomedical Sciences, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada
推荐引用方式
GB/T 7714
He YH,Li W,Li Y,et al. Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes[J]. PLOS ONE,2014,9(10):e110711.
APA He YH,Li W,Li Y,Zhang SC,Wang YW[*],&Sun CH[*].(2014).Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes.PLOS ONE,9(10),e110711.
MLA He YH,et al."Ursolic Acid Increases Glucose Uptake through the PI3K Signaling Pathway in Adipocytes".PLOS ONE 9.10(2014):e110711.

入库方式: OAI收割

来源:昆明动物研究所

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