Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews
文献类型：期刊论文
| 作者 | Li GP1,2; Luo HR3; Sun GT2; Wu GS2,4; Chen BS[*]2; Wang Y1; Man Y1; Wang S1; Li J[*]1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2013 |
| 卷号 | 40期号:9页码:5429-5438 |
| 关键词 | Recombinant protein Tree shrew Triglyceride Apolipoprotein AV cDNA sequence HDL-C |
| 通讯作者 | lijian@bjhmoh.cn ; bschen@ibms.pumc.edu.cn |
| 英文摘要 | Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to atherosclerosis, we performed apoAV cloning and characterization. The full-length cDNA of apoAV was identified using SMART-RACE. ApoAV cDNA sequence revealed two transcripts, 1,948 and 1,397 base pairs, due to alternative polyadenylation. These two transcripts share the same open reading frame (ORF), which encodes a 369-amino acid protein with high identity to human apoAV (75 %), including a 23-amino acid N-terminal signal peptide. ApoAV is expressed exclusively in the liver. Mature apoAV was expressed in E. coli BL21(DE3) and purified by Ni-chelated resin. Lipoprotein lipase activity was significantly stimulated by this recombinant protein. The full-length ORF of apoAV was cloned into pDsRed-monomer-N1 vector with a red fluorescent protein tag and was primarily localized in cytoplasm of hepG2 cells. The successful cloning, expression and localization of apoAV in tree shrew has laid down the foundation for further investigation on its structure and functions. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Grants from National Basic Research Program of China (2012CB517502 and 2006CB503801), the National Natural Science Foundation of China (No. 81270948, 81070634, 81170381), and Beijing Hospital Youth Initial Funding (BJ-2011-30). |
| 语种 | 英语 |
| WOS记录号 | WOS:000323504000021 |
| 公开日期 | 2014-10-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8082]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China 2.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Institute of Basic Medical Sciences, National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China 3.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China 4.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Li GP,Luo HR,Sun GT,et al. Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews[J]. MOLECULAR BIOLOGY REPORTS,2013,40(9):5429-5438. |
| APA | Li GP.,Luo HR.,Sun GT.,Wu GS.,Chen BS[*].,...&Li J[*].(2013).Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews.MOLECULAR BIOLOGY REPORTS,40(9),5429-5438. |
| MLA | Li GP,et al."Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews".MOLECULAR BIOLOGY REPORTS 40.9(2013):5429-5438. |
入库方式： OAI收割
来源：昆明动物研究所
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