热门
Drug Absorption Efficiency in Caenorhbditis elegans Delivered by Different Methods
文献类型：期刊论文
| 作者 | Zheng SQ1,2; Ding AJ1,2; Li GP3; Wu GS4; Luo HR[*]1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:2页码:e56877 |
| 通讯作者 | luohuairong@mail.kib.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Background: Caenorhbditis elegans has being vigorously used as a model organism in many research fields and often accompanied by administrating with various drugs. The methods of delivering drugs to worms are varied from one study to another, which make difficult in comparing results between studies. Methodology/Principal Findings: We evaluated the drug absorption efficiency in C. elegans using five frequently used methods with resveratrol with low aqueous solubility and water-soluble 5-Fluoro-2'-deoxyuridine (FUDR) as positive compounds. The drugs were either applied to the LB medium with bacteria OP50, before spreading onto Nematode Growth Medium (NGM) plates (LB medium method), or to the NGM with live (NGM live method) or dead bacteria (NGM dead method), or spotting the drug solution to the surface of plates directly (spot dead method), or growing the worms in liquid medium (liquid growing method). The concentration of resveratrol and FUDR increased gradually within C. elegans and reached the highest during 12 hours to one day and then decreased slowly. At the same time point, the higher the drug concentration, the higher the metabolism rate. The drug concentrations in worms fed with dead bacteria were higher than with live bacteria at the same time point. Consistently, the drug concentration in medium with live bacteria decreased much faster than in medium with dead bacteria, reach to about half of the original concentration within 12 hours. Conclusion: Resveratrol with low aqueous solubility and water-soluble FUDR have the same absorption and metabolism pattern. The drug metabolism rate in worms was both dosage and time dependent. NGM dead method and liquid growing method achieved the best absorption efficiency in worms. The drug concentration within worms was comparable with that in mice, providing a bridge for dose translation from worms to mammals. |
| 收录类别 | SCI |
| 资助信息 | This work was partially supported by the Yunnan provincial government (20080A007), the 100 Talents Program of the Chinese Academy of Sciences, and the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany (P2008-ZZ21 and T2009-KF05). |
| 语种 | 英语 |
| WOS记录号 | WOS:000316849500039 |
| 公开日期 | 2014-10-17 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8094]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China 2.University of Chinese Academy of Sciences, Beijing, China 3.The key laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, China 4.Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Zheng SQ,Ding AJ,Li GP,et al. Drug Absorption Efficiency in Caenorhbditis elegans Delivered by Different Methods[J]. PLOS ONE,2013,8(2):e56877. |
| APA | Zheng SQ,Ding AJ,Li GP,Wu GS,&Luo HR[*].(2013).Drug Absorption Efficiency in Caenorhbditis elegans Delivered by Different Methods.PLOS ONE,8(2),e56877. |
| MLA | Zheng SQ,et al."Drug Absorption Efficiency in Caenorhbditis elegans Delivered by Different Methods".PLOS ONE 8.2(2013):e56877. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。