HSV-1 infection of tree shrews differs from that of mice in the severity of acute infection and viral transcription in the peripheral nervous system
文献类型：期刊论文
| 作者 | Li LH1; Li ZR1; Wang RL1; Wang R1; Xiao Y1; Han HB2; Lang FC1; Li X1; Xia YJ3,4; Gao F5 |
| 刊名 | Journal of Virology
 |
| 出版日期 | 2015 |
| 卷号 | 90期号:2页码:790-804 |
| 通讯作者 | zhoujm@mail.kiz.ac.cn |
| 英文摘要 | Studies of HSV infections of humans are limited by the use of rodent models such as mice, rabbits and guinea pigs. Tree shrews (Tupaia belangeri chinensis) are small mammals indigenous to southwest Asia. At behavioral, anatomical, genomic and evolutional levels, tree shrews are much closer to primates than rodents, and tree shrews are susceptible to HSV infection. Thus we have studied HSV-1 infection in the tree shrew trigeminal ganglion following ocular inoculation. In situ hybridization, PCR and qRT-PCR analyses confirm that HSV-1 latently infects neurons of the trigeminal ganglion (TG). When explant co-cultivation of trigeminal ganglia was performed, the virus was recovered after 5 days of co-cultivation with high efficiency. Swabbing of the cornea of latently infected tree shrews revealed that tree shrews shed virus spontaneously at low frequencies. However, tree shrews differ significantly from mice in the expression of key HSV-1 genes including ICP0, ICP4 and LAT. In acutely infected tree shrew TGs, no detectable level of ICP4 was seen, suggesting the absence of, or a very weak, acute infection compared to the mouse. Immunofluorescent staining with ICP4 monoclonal antibody, and immunohistochemistry detection by HSV-1 polyclonal antibodies, showed a lack of viral proteins in tree shrew TGs during both acute and latent phases of infection. Cultivation of supernatant from homogenized, acutely infected TGs with RS1 cells also exhibited an absence of infectious HSV-1 from tree shrew TGs. We conclude that the tree shrew has an undetectable, or a much weaker, acute infection in the TGs. Interestingly, compared to mice, tree shrew TGs express high level of ICP0 transcript in addition to LAT during latency. However the ICP0 transcript remained nuclear and no ICP0 protein could be seen in during the course of mouse and tree shrew TG infections. Taken together, these observations suggest that the tree shrew TG infection differs significantly from the existing rodent models. |
| 收录类别 | SCI |
| 资助信息 | National Science Foundation of China provided funding to Jumin Zhou under grant number NSFC81471966. A Common Project of Panzhihua, Science and Technology Bureau of China provided funding to Hongbo Han under grant number 2012CY-S-22(9). A Visiting Professorship for Senior International Scientists from CAS provided funding to Nigel W. Fraserundergrantnumber2012T1S0001.KunmingInstituteofZoology, Chinese Academy of Sciences provided funding to Jumin Zhou under grant number Y102421081. Yunnan Provincial Government provided funding to Jumin Zhou under grant numbers 2011HA005 and 2013FA051. Chinese Academy of Sciences (CAS) provided funding to Jumin Zhou under grant number KSCXZ-EW-BR-6. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9470]  |
| 专题 | 昆明动物研究所_基因调控与表达遗传 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.School of Life Sciences, Panzhihua College, Panzhihua, Sichuan, China 3.; Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 4.; Center for Drug Safety Evaluation, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 5.Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China 6.Department of Viral Immunology, Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming, Kunming, China 7.Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA |
| 推荐引用方式 GB/T 7714 | Li LH,Li ZR,Wang RL,et al. HSV-1 infection of tree shrews differs from that of mice in the severity of acute infection and viral transcription in the peripheral nervous system[J]. Journal of Virology,2015,90(2):790-804. |
| APA | Li LH.,Li ZR.,Wang RL.,Wang R.,Xiao Y.,...&Fraser NW.(2015).HSV-1 infection of tree shrews differs from that of mice in the severity of acute infection and viral transcription in the peripheral nervous system.Journal of Virology,90(2),790-804. |
| MLA | Li LH,et al."HSV-1 infection of tree shrews differs from that of mice in the severity of acute infection and viral transcription in the peripheral nervous system".Journal of Virology 90.2(2015):790-804. |
入库方式： OAI收割
来源：昆明动物研究所
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