Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes
文献类型：期刊论文
| 作者 | Li X1,2; Zhao L1,2; Jiang HF1,2; Wang W[*]1 |
| 刊名 | JOURNAL OF MOLECULAR EVOLUTION
 |
| 出版日期 | 2009 |
| 卷号 | 68期号:1页码:56-65 |
| 关键词 | Chimeric RNAs Short homologous sequences trans-Splicing Transcriptional slippage Complexity of transcriptome |
| ISSN号 | 0022-2844 |
| 通讯作者 | wwang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Chimeric RNAs have been reported in varieties of organisms and are conventionally thought to be produced by trans-splicing of two or more distinct transcripts. Here, we conducted a large-scale search for chimeric RNAs in the budding yeast, fruit fly, mouse, and human. Thousands of chimeric transcripts were identified in these organisms except in yeast, in which five chimeric RNAs were observed. RT-PCR experiments for a sample of yeast and fly chimeric transcripts using specific primers show that about one-third of these chimeric RNAs can be reproduced. The results suggest that at least a considerable amount of chimeric RNAs is unlikely from aberrant transcription or splicing, and thus formation of chimeric RNAs is probably a widespread process and can greatly contribute to the complexity of the transcriptome and proteome of organisms. However, only a small fraction (< 20%) of these chimeric RNAs has GU-AG at the junction sequences which fits the classical trans-splicing model. In contrast, we observed that about half of the chimeric RNAs have short homologous sequences (SHSs) at the junction sites of the source sequences. Our sequence mutation experiments in yeast showed that disruption of SHSs resulted in the disappearance of the corresponding chimeric RNAs, suggesting that SHSs are essential for generating this kind of chimeric RNA. In addition to the classical trans-splicing model, we propose a new model, the transcriptional slippage model, to explain the generation of those chimeric RNAs synthesized from templates with SHSs. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4853]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.CAS-Max Planck Junior Research Group on Evolutionary Genomics, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming 650223, China 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Li X,Zhao L,Jiang HF,et al. Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes[J]. JOURNAL OF MOLECULAR EVOLUTION,2009,68(1):56-65. |
| APA | Li X,Zhao L,Jiang HF,&Wang W[*].(2009).Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes.JOURNAL OF MOLECULAR EVOLUTION,68(1),56-65. |
| MLA | Li X,et al."Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes".JOURNAL OF MOLECULAR EVOLUTION 68.1(2009):56-65. |
入库方式： OAI收割
来源：昆明动物研究所
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