中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 昆明动物研究所
  3. 昆明动物研究所
  4. 基因起源组
Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca)

文献类型：期刊论文

作者Zheng Y1,2,3; Cai J4; Li JW3,6; Li BW3; Lin RM3; Tian F3; Wang XL3; Wang J[*]3,5
刊名SCIENCE CHINA-LIFE SCIENCES
出版日期2010
卷号53期号:1页码:107-111
关键词giant panda BAC sequencing assembly annotation evolution
ISSN号1674-7305
通讯作者wangj@genomics.org.cn
合作状况其它
英文摘要A 10-fold BAC library for giant panda was constructed and nine BACs were selected to generate finish sequences. These BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of giant panda newly generated by the Illumina GA sequencing technology. Complete sanger sequencing, assembly, annotation and comparative analysis were carried out on the selected BACs of a joint length 878 kb. Homologue search and de novo prediction methods were used to annotate genes and repeats. Twelve protein coding genes were predicted, seven of which could be functionally annotated. The seven genes have an average gene size of about 41 kb, an average coding size of about 1.2 kb and an average exon number of 6 per gene. Besides, seven tRNA genes were found. About 27 percent of the BAC sequence is composed of repeats. A phylogenetic tree was constructed using neighbor-join algorithm across five species, including giant panda, human, dog, cat and mouse, which reconfirms dog as the most related species to giant panda. Our results provide detailed sequence and structure information for new genes and repeats of giant panda, which will be helpful for further studies on the giant panda.
收录类别SCI
原文出处201053107.pdf
语种英语
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/4865]  
专题昆明动物研究所_基因起源组
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Beijing Institute of Genomics, Chinese Academy of Sciences (CAS), Beijing 100029, China
2.College of Life Science, Graduate University of Chinese Academy of Sciences, Beijing 100049, China
3.Beijing Genomics Institute at Shenzhen, Shenzhen 518083, China
4.CAS-Max Planck Junior Research Group on Evolutionary Genomics, State Key Laboratory of Genetic Resources and Evolution, Kunming In- stitute of Zoology, Chinese Academy of Sciences (CAS), Kunming 650223, China
5.Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark
6.School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China
推荐引用方式
GB/T 7714		 Zheng Y,Cai J,Li JW,et al. Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca)[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(1):107-111.
APA Zheng Y.,Cai J.,Li JW.,Li BW.,Lin RM.,...&Wang J[*].(2010).Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca).SCIENCE CHINA-LIFE SCIENCES,53(1),107-111.
MLA Zheng Y,et al."Sequencing, annotation and comparative analysis of nine BACs of giant panda (Ailuropoda melanoleuca)".SCIENCE CHINA-LIFE SCIENCES 53.1(2010):107-111.

入库方式： OAI收割

来源：昆明动物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。