The DNA Methylome of Human Peripheral Blood Mononuclear Cells
文献类型：期刊论文
| 作者 | Li YR1; Zhu JD2,3; Tian G1,4,5; Li N1; Li QB1; Ye MZ1; Zheng HC1; Yu J2; Wu HL1; Sun JH1 |
| 刊名 | PLOS BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 8期号:11页码:e1000533 |
| 通讯作者 | s.beck@ucl.ac.uk ; wangj@genomics.org.cn ; zhangxq@genomics.org.cn |
| 合作状况 | 其它 |
| 英文摘要 | DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and <0.2% of non-CpG sites were methylated, demonstrating that non-CpG cytosine methylation is minor in human PBMC. Analysis of the PBMC methylome revealed a rich epigenomic landscape for 20 distinct genomic features, including regulatory, protein-coding, non-coding, RNA-coding, and repeat sequences. Integration of our methylome data with the YH genome sequence enabled a first comprehensive assessment of allele-specific methylation (ASM) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hDMRs) covering 287 genes. Of these, 76 genes had hDMRs within 2 kb of their transcriptional start sites of which >80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies. |
| 收录类别 | SCI |
| 资助信息 | This project was supported by the National Natural Science Foundation of China (30725008; 30890032;30811130531), the Chinese 863 program (2006AA02Z177, 2006AA02A301), the Chinese Academy of Science (GJHZ0701-6), the Shenzhen Municipal Government of China (grants JC200903190772A, CXB200903110066A, ZYC200903240077A, ZYC200903240076A and ZYC200903240080A), the Danish Platform for Integrative Biology and the Ole Romer grant from the Danish Natural Science Research Council. This project was also funded by the Yantian District local government of Shenzhen. SB was supported by The Wellcome Trust (grant 084071) and is recipient of a Royal Society Wolfson Research Merit Award. JZ is supported by the funds from National Science Foundation(90919024, and 30921140312), National Research Program for Basic Research(2009CB825606, 2009CB825607 and 2010CB912802), and Shanghai Science Foundation(09JC141300). |
| 语种 | 英语 |
| 公开日期 | 2010-12-21 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6554]  |
| 专题 | 昆明动物研究所_基因起源组 |
| 作者单位 | 1.BGI-Shenzhen, Shenzhen, Guangdong, China 2.Cancer Epigenetics and Gene Therapy Program, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai, China 3.Epigentic Laboratory, Bio-X Center, Shanghai JiaoTong University, Shanghai, China 4.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China, 5.The Graduate University of Chinese Academy of Sciences, Beijing, China 6.School of Bioscience and Biotechnology, South China University of Technology, Guangzhou, China 7.Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, China 8.Department of Biology, University of Copenhagen, Copenhagen, Denmark, 9.Institute of Human Genetics, University of Aarhus, Aarhus, Denmark 10.Institute of Genetics and Developmental Biology, Chinese Academy of Human Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | Li YR,Zhu JD,Tian G,et al. The DNA Methylome of Human Peripheral Blood Mononuclear Cells[J]. PLOS BIOLOGY,2010,8(11):e1000533. |
| APA | Li YR.,Zhu JD.,Tian G.,Li N.,Li QB.,...&Zhang XQ[*].(2010).The DNA Methylome of Human Peripheral Blood Mononuclear Cells.PLOS BIOLOGY,8(11),e1000533. |
| MLA | Li YR,et al."The DNA Methylome of Human Peripheral Blood Mononuclear Cells".PLOS BIOLOGY 8.11(2010):e1000533. |
入库方式： OAI收割
来源：昆明动物研究所
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