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Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
文献类型：期刊论文
| 作者 | Hou Y1,2,3; Song LT1,4,5,6; Zhu P7; Zhang B1; Tao Y1; Xu X1; Wang W5; Li YR[*]1; Zhang XQ[*]1; Wang J[*]1,9,10 |
| 刊名 | CELL
 |
| 出版日期 | 2012 |
| 卷号 | 148期号:5页码:873-885 |
| 其他题名 | wangj@genomics.org.cn; liyr@genomics.org.cn; zhangxq@genomics.org.cn; wangj@genomics.org.cn |
| 通讯作者 | wangj@genomics.org.cn ; liyr@genomics.org.cn ; zhangxq@genomics.org.cn ; wangj@genomics.org.cn |
| 合作状况 | 其它 |
| 英文摘要 | Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient. The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution. We further identified essential thrombocythemia (ET)-related candidate mutations such as SESN2 and NTRK1, which may be involved in neoplasm progression. This pilot study allowed the initial characterization of the disease-related genetic architecture at the single-cell nucleotide level. Further, we established a single-cell sequencing method that opens the way for detailed analyses of a variety of tumor types, including those with high genetic complex between patients |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a National Basic Research Program of China (973 program number 2011CB809202;2011CB809203), the Chinese 863 program(numbers2009AA022707 and 2012AA02A201),theShenzhen Munic- ipal Government of China (grant ZYC201005250020A), the Key Laboratory Project Supported by Shenzhen City (grants CX B200903110066A and CXB201108250096A), and Shenzhen Key Laboratory of Gene Bank for NationalLifeScience.ThisprojectwasalsosupportedbygrantsfromtheInno- vative Research Team Project of Guangdong and the Guangdong Enterprise Key Laboratory of Human Disease Genomics. We also acknowledge the Ole Rømer grant from the Danish Natural Science Research Council, the Danish National Research Foundation, the National Natural Science Foundation of China, and funds from the Shenzhen Municipal Government and the Local Government of Yantian District of Shenzhen. |
| 语种 | 英语 |
| WOS记录号 | WOS:000300985000009 |
| 公开日期 | 2012-04-20 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6926]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.BGI-Shenzhen, Shenzhen, 518083, China 2.State Key Laboratory of Bioelectronics 3.School of Biological Science and Medical Engineering Southeast University, Nanjing 210096, China 4.College of Life Sciences, Wuhan University, Wuhan 430072, China 5.CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan 650223, China 6.Graduate School of Chinese Academy of Sciences, Beijing 100049, China 7.Department of Hematology, Peking University First Hospital, Beijing 100034, China 8.Pfizer Inc., San Diego, CA 92121, USA 9.Department of Biology 10.The Novo Nordisk Foundation Center for Basic Metabolic Research University of Copenhagen, DK-1165 Copenhagen, Denmark |
| 推荐引用方式 GB/T 7714 | Hou Y,Song LT,Zhu P,et al. Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm[J]. CELL,2012,148(5):873-885. |
| APA | Hou Y.,Song LT.,Zhu P.,Zhang B.,Tao Y.,...&Wang J[*].(2012).Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm.CELL,148(5),873-885. |
| MLA | Hou Y,et al."Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm".CELL 148.5(2012):873-885. |
入库方式： OAI收割
来源：昆明动物研究所
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