Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication
文献类型：期刊论文
| 作者 | Xiang H1; Li X1; Dai FY2; Xu X3; Tan AJ4; Chen L1; Zhang GJ3; Ding Y1; Li QY3; Lian JM3 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2013 |
| 卷号 | 14期号:X页码:e646 |
| 关键词 | dnmt1 Comparative methylomics Silkworm Domestication |
| 通讯作者 | wangj@genomics.org.cn ; wwang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Background: In contrast to wild species, which have typically evolved phenotypes over long periods of natural selection, domesticates rapidly gained human-preferred agronomic traits in a relatively short-time frame via artificial selection. Under domesticated conditions, many traits can be observed that cannot only be due to environmental alteration. In the case of silkworms, aside from genetic divergence, whether epigenetic divergence played a role in domestication is an unanswered question. The silkworm is still an enigma in that it has two DNA methyltransferases (DNMT1 and DNMT2) but their functionality is unknown. Even in particular the functionality of the widely distributed DNMT1 remains unknown in insects in general. Results: By embryonic RNA interference, we reveal that knockdown of silkworm Dnmt1 caused decreased hatchability, providing the first direct experimental evidence of functional significance of insect Dnmt1. In the light of this fact and those that DNA methylation is correlated with gene expression in silkworms and some agronomic traits in domesticated organisms are not stable, we comprehensively compare silk gland methylomes of 3 domesticated (Bombyx mori) and 4 wild (Bombyx mandarina) silkworms to identify differentially methylated genes between the two. We observed 2-fold more differentiated methylated cytosinces (mCs) in domesticated silkworms as compared to their wild counterparts, suggesting a trend of increasing DNA methylation during domestication. Further study of more domesticated and wild silkworms narrowed down the domesticates' epimutations, and we were able to identify a number of differential genes. One such gene showing demethyaltion in domesticates correspondently displays lower gene expression, and more interestingly, has experienced selective sweep. A methylation-increased gene seems to result in higher expression in domesticates and the function of its Drosophila homolog was previously found to be essential for cell volume regulation, indicating a possible correlation with the enlargement of silk glands in domesticated silkworms. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a key grant of National Natural Science Foundation of China (No. 90919056) and 973 program (2013CB835200) to W.W, a key grant of West Light Foundation of the Chinese Academy of Sciences and a special fellowship of China Postdoctoral Science Foundation to H. X, a CAS-Max Planck Society Fellowship to W.W. and a 973 Program (No. 2012CB114600) to F.Y.D. |
| 语种 | 英语 |
| WOS记录号 | WOS:000326171800003 |
| 公开日期 | 2013-11-25 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7712]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiaochang Road, Kunming, Yunnan Province 650223, Chin 2.State Key Laboratory of Silkworm Genome Biology, Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, No.2 Tiansheng Road, Chongqing, BeiBei District 400715, China 3.BGI-Shenzhen, Shenzhen, China, Beishan Industrial Zone, Shenzhen, Yantian District 518083, China 4.Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China. 5.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang H,Li X,Dai FY,et al. Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication[J]. BMC GENOMICS,2013,14(X):e646. |
| APA | Xiang H.,Li X.,Dai FY.,Xu X.,Tan AJ.,...&Wang W[*].(2013).Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication.BMC GENOMICS,14(X),e646. |
| MLA | Xiang H,et al."Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication".BMC GENOMICS 14.X(2013):e646. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。