Advances in genome editing technology and its promising application in evolutionary and ecological studies
文献类型：期刊论文
| 作者 | Chen L1; Tang LY2; Xiang H1; Jin LJ3; Li QY3; Dong Y4; Wang W[*]1,4; Zhang GJ[*]3 |
| 刊名 | GigaScience
 |
| 出版日期 | 2014 |
| 卷号 | 3期号:X页码:e24 |
| 关键词 | Domestication Genetic innovations Genetic modification Life-environment interaction |
| 通讯作者 | wwang@mail.kiz.ac.cn ; zhanggj@genomics.cn |
| 合作状况 | 其它 |
| 英文摘要 | Genetic modification has long provided an approach for "reverse genetics", analyzing gene function and linking DNA sequence to phenotype. However, traditional genome editing technologies have not kept pace with the soaring progress of the genome sequencing era, as a result of their inefficiency, time-consuming and labor-intensive methods. Recently, invented genome modification technologies, such as ZFN (Zinc Finger Nuclease), TALEN (Transcription Activator-Like Effector Nuclease), and CRISPR/Cas9 nuclease (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nuclease) can initiate genome editing easily, precisely and with no limitations by organism. These new tools have also offered intriguing possibilities for conducting functional large-scale experiments. In this review, we begin with a brief introduction of ZFN, TALEN, and CRISPR/Cas9 technologies, then generate an extensive prediction of effective TALEN and CRISPR/Cas9 target sites in the genomes of a broad range of taxonomic species. Based on the evidence, we highlight the potential and practicalities of TALEN and CRISPR/Cas9 editing in non-model organisms, and also compare the technologies and test interesting issues such as the functions of candidate domesticated, as well as candidate genes in life-environment interactions. When accompanied with a high-throughput sequencing platform, we forecast their potential revolutionary impacts on evolutionary and ecological research, which may offer an exciting prospect for connecting the gap between DNA sequence and phenotype in the near future. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a 973 program (2013CB835200) to W.W, a key grant of West Light Foundation of the Chinese Academy of Sciences to H.X. |
| 语种 | 英语 |
| 公开日期 | 2015-06-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8363]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences and University of Chinese Chen et al. GigaScience 2014, 3:24 Page 7 of 10 http://www.gigasciencejournal.com/content/3/1/24 Academy of Sciences, No. 32 Jiaochang Donglu, Kunming, Yunnan 650223, China 2.College of Life Sciences, Wuhan University, Wuhan 430072, China. 3.China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen 518083, China 4.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Chen L,Tang LY,Xiang H,et al. Advances in genome editing technology and its promising application in evolutionary and ecological studies[J]. GigaScience,2014,3(X):e24. |
| APA | Chen L.,Tang LY.,Xiang H.,Jin LJ.,Li QY.,...&Zhang GJ[*].(2014).Advances in genome editing technology and its promising application in evolutionary and ecological studies.GigaScience,3(X),e24. |
| MLA | Chen L,et al."Advances in genome editing technology and its promising application in evolutionary and ecological studies".GigaScience 3.X(2014):e24. |
入库方式： OAI收割
来源：昆明动物研究所
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