Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
文献类型：期刊论文
| 作者 | Li YR1; Xu X1; Tsang S10; Wang W2; Lu ZH5,6; Zhang XQ1; Bolund L1,15; Kristiansen K1,16; Song LT1,2,3,4; Wang J1 |
| 刊名 | GIGASCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 1期号:X页码:e12 |
| 关键词 | Single-cell exome sequencing Bladder cancer Tumor evolution Population genetics |
| 通讯作者 | yanghm@genomics.cn ; deanm@mail.nih.gov ; wangj@genomics.cn |
| 合作状况 | 其它 |
| 英文摘要 | Background: Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level. Results: We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively. Conclusions: This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by a National Basic Research Program of China (973 program no.2011CB809202;2011CB809203), the Chinese 863 program (no.2009AA022707), the Shenzhen Municipal Government of China (grants ZYC201005250020A ), the Key Laboratory Project Supported by Shenzhen City (grants CX B200903 110066A; CXB201108250096A), and Shenzhen Key Laboratory of Gene Bank for National Life Science. This project was also supported by a grant from the Innovative Research Team Project of GuangDong and the Guangdong Enterprise Key Laboratory of Human Disease Genomics, and in part by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. We also thank the Danish Natural Science Research Council for the Ole Rømer grant and the Shenzhen Municipal Government and the Local Government of Yantian District of Shenzhen for providing funding and an in part by the Intramural Research Program of the National Cancer Institute, National Institutes of Health, USA. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10400]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.BGI Shenzhen, Beishan Ind Zone, Shenzhen 518083, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, CAS Max Planck Jr Res Grp, Kunming 650223, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Sch Biosci & Biotechnol, Guangzhou 510006, Guangdong, Peoples R China 5.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen PKU HKUST Med Ctr, Inst Urol,Shenzhen Hosp, Shenzhen 518036, Peoples R China 6.Longgang Cent Hosp, Dept Urol, Shenzhen 518116, Peoples R China 7.Univ Aarhus, Inst Human Genet, DK-8100 Aarhus, Denmark 8.Univ Copenhagen, Novo Nordisk Fdn Ctr Basic Metab Res, DK-2200 Copenhagen, Denmark 9.Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark 10.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li YR,Xu X,Tsang S,et al. Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer[J]. GIGASCIENCE,2012,1(X):e12. |
| APA | Li YR.,Xu X.,Tsang S.,Wang W.,Lu ZH.,...&Li ZS.(2012).Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer.GIGASCIENCE,1(X),e12. |
| MLA | Li YR,et al."Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer".GIGASCIENCE 1.X(2012):e12. |
入库方式： OAI收割
来源：昆明动物研究所
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