Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer
文献类型：期刊论文
| 作者 | Li H1,2; Wang G3; Hao ZQ4,5; Zhang GZ6,7; Qing YB2,6; Liu SH4; Qing LL6; Pan WR2; Zhao HY[*]1,6; Wang W[*]5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:X页码:e33675 |
| 通讯作者 | hyzhao2000@126.com ; wwang@mail.kiz.ac.cn ; hongjiangwei@126.com |
| 合作状况 | 其它 |
| 英文摘要 | Transgenic sheep can be used to achieve genetic improvements in breeds and as an important large-animal model for biomedical research. In this study, we generated a TALEN plasmid specific for ovine MSTN and transfected it into fetal fibroblast cells of STH sheep. MSTN biallelic-KO somatic cells were selected as nuclear donor cells for SCNT. In total, cloned embryos were transferred into 37 recipient gilts, 28 (75.7%) becoming pregnant and 15 delivering, resulting in 23 lambs, 12 of which were alive. Mutations in the lambs were verified via sequencing and T7EI assay, and the gene mutation site was consistent with that in the donor cells. Off-target analysis was performed, and no off-target mutations were detected. MSTN KO affected the mRNA expression of MSTN relative genes. The growth curve for the resulting sheep suggested that MSTN KO caused a remarkable increase in body weight compared with those of wild-type sheep. Histological analyses revealed that MSTN KO resulted in muscle fiber hypertrophy. These findings demonstrate the successful generation of MSTN biallelic-KO STH sheep via gene editing in somatic cells using TALEN technology and SCNT. These MSTN mutant sheep developed and grew normally, and exhibited increased body weight and muscle growth. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Genetically Modified Organisms Breeding Major Projects (Grant No. 2014ZX08010002-005, Wen Wang, 2016ZX08009-003-006, Hong-Jiang Wei), the key project of Chinese Academy of Sciences (CAS) (Grant No. KSZD-EW-Z-005-003, Wen Wang), the National Natural Science Foundation of China (Grant No. 31360549, Hong-Jiang Wei), Strategic Priority Research Program (B) of CAS (XDB13000000, Wen Wang), and the Natural Science Foundation Key Project of Yunnan Province (Grant No. 2013FA016, Hong-Jiang Wei). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10506]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China 2.College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China 3.College of Hetao, Bayannaoer 015000, China 4.Inner Mongolia Zhong-Ke-Zheng-Biao Biotech Co., Ltd, Bayannaoer 015400, China 5.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 6.Reproductive & Developmental Laboratory, Southwest China Biodiversity Laboratory, Kunming 650203, China 7.Key Laboratory Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China 8.Bayannaoer Livestock Improvement Station, Bayannaoer 015000, China |
| 推荐引用方式 GB/T 7714 | Li H,Wang G,Hao ZQ,et al. Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer[J]. SCIENTIFIC REPORTS,2016,6(X):e33675. |
| APA | Li H.,Wang G.,Hao ZQ.,Zhang GZ.,Qing YB.,...&Wei HJ[*].(2016).Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer.SCIENTIFIC REPORTS,6(X),e33675. |
| MLA | Li H,et al."Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer".SCIENTIFIC REPORTS 6.X(2016):e33675. |
入库方式： OAI收割
来源：昆明动物研究所
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