Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus
文献类型：期刊论文
| 作者 | Tan XJ1; Qin N2; Wu CY4; Sheng JY1; Yang R1; Zheng BW2; Ma ZS5; Liu L2; Peng XH[*]3; Jia AQ[*]1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:X页码:e11997 |
| 通讯作者 | xinhpeng@mail.njust.edu.cn ; jiaaiqun@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus. |
| 收录类别 | SCI |
| 资助信息 | The study was supported by the National Natural Science Foundation of China (31170131, 31070312, 81301475) and Jiangsu Qinglan Project. We thank Dr. Liyan Ping from Harvard University, USA and Dr. Heiko Maischak from Max Planck Institute for Chemical Ecology, Germany for polishing the paper, and thank Dr. Jing Chen from Zhengjiang University, China for guiding qPCR experiments. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9373]  |
| 专题 | 昆明动物研究所_计算生物与生物信息学 |
| 作者单位 | 1.School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China 2.State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China 3.School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China 4.Realbio Genomics Institute, Shanghai 200050, China 5.Kunming Institute of Zoology, Chinese Academy of Science, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Tan XJ,Qin N,Wu CY,et al. Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus[J]. SCIENTIFIC REPORTS,2015,5(X):e11997. |
| APA | Tan XJ.,Qin N.,Wu CY.,Sheng JY.,Yang R.,...&Jia AQ[*].(2015).Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.SCIENTIFIC REPORTS,5(X),e11997. |
| MLA | Tan XJ,et al."Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus".SCIENTIFIC REPORTS 5.X(2015):e11997. |
入库方式： OAI收割
来源：昆明动物研究所
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