Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing
文献类型：期刊论文
| 作者 | Zhao YQ[*]1; Wang YJ2; Zou L3; Huang JF[*]1,4 |
| 刊名 | FEBS Open Bio
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:4页码:264-275 |
| 关键词 | RNA sequencing Saccharomyces species metabolic engineering metabolic network |
| 通讯作者 | zhaoyq@mail.kiz.ac.cn ; huangjf@mail.kiz.ac.cn |
| 英文摘要 | One practical application of genome-scale metabolic reconstructions is to interrogate multispecies relationships. Here, we report a consensus metabolic model in four yeast species (Saccharomyces cerevisiae, S. paradoxus, S. mikatae, and S. bayanus) by integrating metabolic network simulations with RNA sequencing (RNA-seq) datasets. We generated high-resolution transcriptome maps of four yeast species through de novo assembly and genome-guided approaches. The transcriptomes were annotated and applied to build the consensus metabolic network, which was verified using independent RNA-seq experiments. The expression profiles reveal that the genes involved in amino acid and lipid metabolism are highly coexpressed. The diverse phenotypic characteristics, such as cellular growth and gene deletions, can be simulated using the metabolic model. We also explored the applications of the consensus model in metabolic engineering using yeast-specific reactions and biofuel production as examples. Similar strategies will benefit communities studying genome-scale metabolic networks of other organisms. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by the grants from National Natural Science Foundation of China (Grant No. 81400084; Grant No. 31123005), National Basic Research Program of China (2013CB835100) |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9930]  |
| 专题 | 昆明动物研究所_结构生物信息学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, China 3.Department of General Surgery, First People’s Hospital of Yunnan Province, Kunming, China 4.Collaborative Innovation Center for Natural Products and Biological Drugs of Yunnan, Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Zhao YQ[*],Wang YJ,Zou L,et al. Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing[J]. FEBS Open Bio,2016,6(4):264-275. |
| APA | Zhao YQ[*],Wang YJ,Zou L,&Huang JF[*].(2016).Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing.FEBS Open Bio,6(4),264-275. |
| MLA | Zhao YQ[*],et al."Reconstruction and applications of consensus yeast metabolic network based on RNA sequencing".FEBS Open Bio 6.4(2016):264-275. |
入库方式： OAI收割
来源：昆明动物研究所
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