Transcriptome Profiles Using Next-Generation Sequencing Reveal Liver Changes in the Early Stage of Diabetes in Tree Shrew (Tupaia belangeri chinensis)
文献类型：期刊论文
| 作者 | Wu XY1,2; Xu HB3,4; Zhang LQ1,5; Li YH1; Wu DD[*]3; Liang B[*]1; Zhang ZG1,5; Chang Q1; Liao SH1,4 |
| 刊名 | Journal of Diabetes Research
 |
| 出版日期 | 2016 |
| 卷号 | 2016期号:X页码:e6238526 |
| 通讯作者 | wudongdong@mail.kiz.ac.cn ; liangb@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Determining the liver changes during the early stages of diabetes is critical to understand the nature of the disease and development of novel treatments for it. Advances in the use of animal models and next-generation sequencing technologies offer a powerful tool in connection between liver changes and the diabetes. Here, we created a tree shrew diabetes model akin to type 1 diabetes by using streptozotocin to induce hyperglycemia and hyperlipidemia. Using RNA-seq, we compiled liver transcriptome profiles to determine the differentially expressed genes and to explore the role of hyperglycemia in liver changes. Our results, respectively, identified 14,060 and 14,335 genes in healthy tree shrews and those with diabetes, with 70 genes differentially expressed between the two groups. Gene orthology and KEGG annotation revealed that several of the main biological processes of these genes were related to translational processes, steroid metabolic processes, oxidative stress, inflammation, and hypertension, all of which are highly associated with diabetes and its complications. These results collectively suggest that STZ induces hyperglycemia in tree shrew and that hyperglycemia induced oxidative stress led to high expression of aldose reductase, inflammation, and even cell death in liver tissues during the early stage of diabetes. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the National Natural Science Foundation of China (U1202223 and Y301261041), the Strate- gic Priority Research Program of the Chinese Academy of Sciences (XDB13030600), the Application Program of Basic Research of Yunnan Province (2014FB180), and the National 863 Project of China (2012AA021801 and 2012AA022402). The authors would also like to thank Andrew Willden of the Kunming Institute of Zoology for assistance with the paper. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9709]  |
| 专题 | 昆明动物研究所_进化与发育转录组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_脂类代谢与疾病 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, Yunnan 650201, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China Yunnan Key Laboratory of Animal Reproduction, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 4.School of Life Sciences, Anhui University, Hefei, Anhui 230601, China 5.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China |
| 推荐引用方式 GB/T 7714 | Wu XY,Xu HB,Zhang LQ,et al. Transcriptome Profiles Using Next-Generation Sequencing Reveal Liver Changes in the Early Stage of Diabetes in Tree Shrew (Tupaia belangeri chinensis)[J]. Journal of Diabetes Research,2016,2016(X):e6238526. |
| APA | Wu XY.,Xu HB.,Zhang LQ.,Li YH.,Wu DD[*].,...&Liao SH.(2016).Transcriptome Profiles Using Next-Generation Sequencing Reveal Liver Changes in the Early Stage of Diabetes in Tree Shrew (Tupaia belangeri chinensis).Journal of Diabetes Research,2016(X),e6238526. |
| MLA | Wu XY,et al."Transcriptome Profiles Using Next-Generation Sequencing Reveal Liver Changes in the Early Stage of Diabetes in Tree Shrew (Tupaia belangeri chinensis)".Journal of Diabetes Research 2016.X(2016):e6238526. |
入库方式： OAI收割
来源：昆明动物研究所
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