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Gut microbiota dysbiosis and bacterial community assembly associated with cholesterol gallstones in large-scale study

文献类型：期刊论文

作者Wu T1; Zhang ZG2; Liu B1; Hou DZ1; Liang Y1; Zhang J[*]3; Shi P[*]2
刊名BMC GENOMICS
出版日期2013
卷号14期号:X页码:e669
关键词Gut microbiota dysbiosis Cholesterol gallstone Bile Bacterial colonization Pyrosequencing
通讯作者jason6677@hotmail.com ; ship@mail.kiz.ac.cn
合作状况其它
英文摘要 Background: Elucidating gut microbiota among gallstone patients as well as the complex bacterial colonization of cholesterol gallstones may help in both the prediction and subsequent lowered risk of cholelithiasis. To this end, we studied the composition of bacterial communities of gut, bile, and gallstones from 29 gallstone patients as well as the gut of 38 normal individuals, examining and analyzing some 299, 217 bacterial 16S rRNA gene sequences from 120 samples. 

Results: First, as compared with normal individuals, in gallstone patients there were significant (P < 0.001) increases of gut bacterial phylum Proteobacteria and decreases of three gut bacterial genera, Faecalibacterium, Lachnospira, and Roseburia. Second, about 70% of gut bacterial operational taxonomic units (OTUs) from gallstone patients were detectable in the biliary tract and bacteria diversity of biliary tract was significantly (P < 0.001) higher than that of gut. Third, analysis of the biliary tract core microbiome (represented by 106 bacteria OTUs) among gallstone patients showed that 33.96% (36/106) of constituents can be matched to known bacterial species (15 of which have publicly available genomes). A genome-wide search of MDR, BSH, bG, and phL genes purpotedly associated with the formation of cholesterol gallstones showed that all 15 species with known genomes (e. g., Propionibacterium acnes, Bacteroides vulgates, and Pseudomonas putida) contained at least contained one of the four genes. This finding could potentially provide underlying information needed to explain the association between biliary tract microbiota and the formation of cholesterol gallstones. 

Conclusions: To the best of our knowledge, this is the first study to discover gut microbiota dysbiosis among gallstone patients, the presence of which may be a key contributor to the complex bacteria community assembly linked with the presence of cholesterol gallstones. Likewise, this study also provides the first large-scale glimpse of biliary tract microbiota potentially associated with cholesterol gallstones. Such a characterization of the biliary tract core microbiome has potentially important biological and medical implications regarding the role of bacteria in the formation cholesterol gallstones.
收录类别SCI
资助信息This research was supported by grants from Natural Science Foundation of Yunnan Province (Grant No. 2010CD147 to TW and Grant No. 2011FA035 to PS) and from both the National Natural Science Foundation of China (NSFC) (Grant No. 31100916 to ZZ) and the Young Talent Project of the Kunming Institute of Zoology, Chinese Academy of Sciences (Grant No. Y106541131 to ZZ) and “A Hundred Talent Program” from Chinese Academy of Sciences to PS.
语种英语
WOS记录号WOS:000326173300002
公开日期2013-11-25
源URL[http://159.226.149.42:8088/handle/152453/7711]  
专题昆明动物研究所_进化与功能基因组学
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
2.State Key Laboratory of Genetic Resources and Evolution, Laboratory of Evolutionary & Functional Genomics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
3.Department of Hepatobiliary Surgery, The Second Clinical College of Kunming Medical University, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan, China
推荐引用方式
GB/T 7714		 Wu T,Zhang ZG,Liu B,et al. Gut microbiota dysbiosis and bacterial community assembly associated with cholesterol gallstones in large-scale study[J]. BMC GENOMICS,2013,14(X):e669.
APA Wu T.,Zhang ZG.,Liu B.,Hou DZ.,Liang Y.,...&Shi P[*].(2013).Gut microbiota dysbiosis and bacterial community assembly associated with cholesterol gallstones in large-scale study.BMC GENOMICS,14(X),e669.
MLA Wu T,et al."Gut microbiota dysbiosis and bacterial community assembly associated with cholesterol gallstones in large-scale study".BMC GENOMICS 14.X(2013):e669.

入库方式： OAI收割

来源：昆明动物研究所

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