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Large-Scale Survey of Gut Microbiota Associated With MHE Via 16S rRNA-Based Pyrosequencing
文献类型：期刊论文
| 作者 | Zhang ZG[*]1; Zhai HQ2; Geng JW[1]2; Yu R1; Ren HQ1; Fan H[*]2; Shi P[*]1 |
| 刊名 | AMERICAN JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 108期号:10页码:1601-1611 |
| 通讯作者 | zhangzhigang@mail.kiz.ac.cn ; fanhong1018@yahoo.com.cn ; ship@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | OBJECTIVES: Elucidating the minimal hepatic encephalopathy (MHE)-associated gut microbiome may help in predicting and lowering the high risk for MHE in patients with cirrhosis. METHODS: Twenty-six MHE patients were recruited and screened from among those with liver cirrhosis without overt hepatic encephalopathy as defined by abnormality seen on two test modalities: number connection test part A and the digit symbol test. Using 26 MHE-matched normal relatives and 25 cirrhotic patients without MHE as controls, by means of 16S ribosomal RNA (rRNA)-based pyrosequencing, we examined and analyzed 241,622 bacterial 16S rDNA gene sequences from feces of 77 subjects. RESULTS: Using multiple comparative analyses, our results found the continuous overrepresentation of two bacterial families, Streptococcaceae and Veillonellaceae, in cirrhotic patients with and without MHE, compared with normal individuals. In addition, we also discovered an MHE-unique interplay pattern of gut microbiota largely influenced by the members of those two families. Following these findings, we further revealed that gut urease-containing bacteria Streptococcus salivarius was absent in the normal group but was present in cirrhotic patients with and without MHE. The abundance of S. salivarius was significantly higher in cirrhotic patients with MHE than in those without (P = 0.030), and the change in the amount of this bacteria was positively correlated with ammonia accumulation (R = 0.58, P = 0.003) in cirrhotic patients with MHE but not in those without. CONCLUSIONS: Gut microbiota dysbiosis may be associated with the presence of MHE in cirrhotic patients, in particular with ammonia-increasing phenotype in MHE. Gut ammonia-increasing bacteria S. salivarius might be expected to be a potential biomarker of ammonia-lowering therapies in cirrhotic patients with MHE. |
| 收录类别 | SCI |
| 资助信息 | Th is research was supported by grants from the National Natural Science Foundation of China (NSFC; grant no. 81260077 to H.F. and grant no. 31100916 to Z.Z.) and from the Natural Science Foundation of Yunnan Province of China (grant no. 2011FA035 to P.S.). |
| 语种 | 英语 |
| WOS记录号 | WOS:000325276100009 |
| 公开日期 | 2014-10-27 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8152]  |
| 专题 | 昆明动物研究所_进化与功能基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences , Kunming , China 2.Department of Gastroenterology, The First People ’ s Hospital of Yunnan Province , Kunming , China |
| 推荐引用方式 GB/T 7714 | Zhang ZG[*],Zhai HQ,Geng JW[1],et al. Large-Scale Survey of Gut Microbiota Associated With MHE Via 16S rRNA-Based Pyrosequencing[J]. AMERICAN JOURNAL OF GASTROENTEROLOGY,2013,108(10):1601-1611. |
| APA | Zhang ZG[*].,Zhai HQ.,Geng JW[1].,Yu R.,Ren HQ.,...&Shi P[*].(2013).Large-Scale Survey of Gut Microbiota Associated With MHE Via 16S rRNA-Based Pyrosequencing.AMERICAN JOURNAL OF GASTROENTEROLOGY,108(10),1601-1611. |
| MLA | Zhang ZG[*],et al."Large-Scale Survey of Gut Microbiota Associated With MHE Via 16S rRNA-Based Pyrosequencing".AMERICAN JOURNAL OF GASTROENTEROLOGY 108.10(2013):1601-1611. |
入库方式： OAI收割
来源：昆明动物研究所
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