Bacteria and Methanogens Differ along the Gastrointestinal Tract of Chinese Roe Deer (Capreolus pygargus)
文献类型：期刊论文
| 作者 | Li ZP1; Zhang ZG[*]2; Xu C1; Zhao JB1; Liu HL1; Fan ZY1; Yang FH1; Wright ADG3; Li GY[*]1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:12页码:e114513 |
| 通讯作者 | zhangzhigang@mail.kiz.ac.cn ; tcslgy@126.com |
| 合作状况 | 其它 |
| 英文摘要 | The current study provides the insight into the bacteria in the gastrointestinal tract (GIT) and methanogens presented in the rumen and cecum of the Chinese roe deer (Capreolus pygargus). The ruminal, ileal, cecal, and colonic contents, as well as feces, were obtained from each of the three, free-range, roe deer ingesting natural pasture after euthanasia. For the bacterial community, a total of 697,031 high-quality 16S rRNA gene sequences were generated using high-throughput sequencing, and assigned to 2,223 core operational taxonomic units (OTUs) (12 bacterial phyla and 87 genera). The phyla Firmicutes (51.2%) and Bacteroidetes (39.4%) were the dominant bacteria in the GIT of roe deer. However, the bacterial community in the rumen was significantly (P<0.01) different from the other sampled regions along the GIT. Secondly, Prevotella spp., Anaerovibrio spp., and unidentified bacteria within the families Veillonellaceae and Paraprevotellaceae were more abundant in the rumen than in the other regions. Unidentified bacteria within the family Enterobacteriaceae, Succinivibrio spp., and Desulfovibrio spp. were more predominant in the colon than in other regions. Unidentified bacteria within the family Ruminococcaceae, and Bacteroides spp. were more prevalent in the ileum, cecum and fecal pellets. For methanogens in the rumen and cecum, a total of 375,647 high quality 16S rRNA gene sequences were obtained and assigned to 113 core OTUs. Methanobrevibacter millerae was the dominant species accounting for 77.3 +/- 7.4 (S. E) % and 68.9 +/- 4.4 (S. E) % of total sequences in the rumen and cecum of roe deer, respectively. However, the abundance of Methanobrevibacter smithii was higher in the rumen than in the cecum (P=0.004). These results revealed that there was intra variation in the bacterial community composition across the GIT of roe deer, and also showed that the methanogen community in the rumen differed from that in the cecum. |
| 收录类别 | SCI |
| 资助信息 | This work is supported by a leading talent and creative team project (20121810), natural science foundation (20130101104JC) from Jilin province, key projects in the national science & technology pillar program (2011BAI03B02), and Foundation of State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (GREKF14-14). |
| 语种 | 英语 |
| WOS记录号 | WOS:000347515300047 |
| 公开日期 | 2015-02-06 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8249]  |
| 专题 | 昆明动物研究所_进化与功能基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Chin 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 3.School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona, United States of America |
| 推荐引用方式 GB/T 7714 | Li ZP,Zhang ZG[*],Xu C,et al. Bacteria and Methanogens Differ along the Gastrointestinal Tract of Chinese Roe Deer (Capreolus pygargus)[J]. PLOS ONE,2014,9(12):e114513. |
| APA | Li ZP.,Zhang ZG[*].,Xu C.,Zhao JB.,Liu HL.,...&Li GY[*].(2014).Bacteria and Methanogens Differ along the Gastrointestinal Tract of Chinese Roe Deer (Capreolus pygargus).PLOS ONE,9(12),e114513. |
| MLA | Li ZP,et al."Bacteria and Methanogens Differ along the Gastrointestinal Tract of Chinese Roe Deer (Capreolus pygargus)".PLOS ONE 9.12(2014):e114513. |
入库方式： OAI收割
来源：昆明动物研究所
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