Genome-wide profiles of methylation, microRNAs, and gene expression in chemoresistant breast cancer
文献类型：期刊论文
| 作者 | He DX1; Gu F2; Gao F3; Hao JJ4; Gong DS3; Gu XT5; Mao AQ5; Jin J[*]5; Fu L[*]2; Ma X[*]5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:X页码:e24706. |
| 通讯作者 | jinjian31@163.com |
| 合作状况 | 其它 |
| 英文摘要 | Cancer chemoresistance is regulated by complex genetic and epigenetic networks. In this study, the features of gene expression, methylation, and microRNA (miRNA) expression were investigated with high-throughput sequencing in human breast cancer MCF-7 cells resistant to adriamycin (MCF-7/ADM) and paclitaxel (MCF-7/PTX). We found that: (1) both of the chemoresistant cell lines had similar, massive changes in gene expression, methylation, and miRNA expression versus chemosensitive controls. (2) Pairwise integration of the data highlighted sets of genes that were regulated by either methylation or miRNAs, and sets of miRNAs whose expression was controlled by DNA methylation in chemoresistant cells. (3) By combining the three sets of high-throughput data, we obtained a list of genes whose expression was regulated by both methylation and miRNAs in chemoresistant cells; (4) Expression of these genes was then validated in clinical breast cancer samples to generate a 17-gene signature that showed good predictive and prognostic power in triple-negative breast cancer patients receiving anthracycline-taxane-based neoadjuvant chemotherapy. In conclusion, our results have generated a new workflow for the integrated analysis of the effects of miRNAs and methylation on gene expression during the development of chemoresistance. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the China National Natural Science Foundation 81572940 and 91439131 to Xin Ma; 31200126 and 31550006 to Dong-Xu He; 81272358 to Feng Gu; the Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province (BK20140004 to Xin Ma); the Program for New Century Excellent Talents in University of The Ministry of Education of China (NCET-12-0880) to Xin Ma; the National High Technology Research and Development Program (863 Program) of China (SQ2015AA020948) to Xin Ma; Fundamental Research Funds for the Central Universities (JUSRP51311A and JUSRP51519) to Xin Ma and Jin Jian. We thank Dr. IC Bruce for reading the manuscript. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9742]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_进化与功能基因组学 |
| 作者单位 | 1.National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China 2.Department of Breast Cancer Pathology and Research Laboratory, State Key Laboratory of Breast Cancer Research, Cancer Institute and Hospital, Tianjin Medical University, Tianjin 300060, PR China 3.Agricultural Genomes Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China 4.State Key Lab of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 5.Department of Cellular and Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China |
| 推荐引用方式 GB/T 7714 | He DX,Gu F,Gao F,et al. Genome-wide profiles of methylation, microRNAs, and gene expression in chemoresistant breast cancer[J]. SCIENTIFIC REPORTS,2016,6(X):e24706.. |
| APA | He DX.,Gu F.,Gao F.,Hao JJ.,Gong DS.,...&Ma X[*].(2016).Genome-wide profiles of methylation, microRNAs, and gene expression in chemoresistant breast cancer.SCIENTIFIC REPORTS,6(X),e24706.. |
| MLA | He DX,et al."Genome-wide profiles of methylation, microRNAs, and gene expression in chemoresistant breast cancer".SCIENTIFIC REPORTS 6.X(2016):e24706.. |
入库方式： OAI收割
来源：昆明动物研究所
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