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| 作者 | Li YH1; Zou CG2; Liu B1; Zhang ZG[*]4; Liu J[*]1; Fu Y3; Li YH1; Zhou Q1
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| 刊名 | BMC GENOMICS
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| 出版日期 | 2016
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| 卷号 | 17期号:X页码:e558 |
| 关键词 | Saliva
Supragingival plaque
Pigment
Caries
Oral microbiota
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| 通讯作者 | zhangzhigang@mail.kiz.ac.cn
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| 英文摘要 |
BACKGROUND:
Black extrinsic discoloration in primary dentition is a common clinical and aesthetic problem that can co-occur with dental caries, the most common oral diseases in childhood. Although the role of bacteria in the formation of pigment and caries in primary dentition is important, their basic features still remain a further mystery.
METHODS:
Using targeted sequencing of the V1-V3 hypervariable regions of bacterial 16S ribosomal RNA (rRNA) genes, we obtained a dataset consisting of 831,381 sequences from 111 saliva samples and 110 supragingival plaque samples from 40 patients with pigment (black extrinsic stain), 20 with caries (obvious decay), and 25 with both pigment and caries and from 26 healthy individuals. We applied a Dirichlet multinomial mixture (DMM)-based community typing approach to investigate oral microbial community types.
RESULTS:
Our results revealed significant structural segregation of microbial communities, as indicated by the identification of two plaque community types (A and B) and three saliva community types (C-E). We found that the independent occurrence of the two plaque community types, A and B, was potentially associated with our oral diseases of interest. For type A, three co-occurring bacterial genus pairs could separately play a potential role in the formation of pigment (Leptotrichia and Fusobacterium), caries (unclassified Gemellales and Granulicatella), and mixed caries and pigment (Streptococcus and Mogibacterium). For type B, three co-occurring bacterial genera (unclassified Clostridiaceae, Peptostreptococcus, and Clostridium) were related to mixed pigment and caries. Three dominant bacterial genera (Selenomonas, Gemella, and Streptobacillus) were linked to the presence of caries.
CONCLUSIONS:
Our study demonstrates that plaque-associated oral microbial communities could majorly contribute to the formation of pigment and caries in primary dentition and suggests potential clinical applications of monitoring oral microbiota as an indicator for disease diagnosis and prognosis.
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| 收录类别 | SCI
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| 资助信息 | This work was funded by support from a grant (No. 2012FB075) from the joint fund for the Yunnan Department of Science and Technology-Kunming Medical University. |
| 语种 | 英语
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| 源URL | [http://159.226.149.26:8080/handle/152453/10362]  |
| 专题 | 昆明动物研究所_进化与功能基因组学
昆明动物研究所_遗传资源与进化国家重点实验室
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| 作者单位 | 1.Department of Preventive & Pediatric Dentistry, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan 650100, China.
2.State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, China
3.Department of Dermatology, Beijing Hospital, Beijing 100730, China.
4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
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推荐引用方式
GB/T 7714 |
Li YH,Zou CG,Liu B,et al. Oral microbial community typing of caries and pigment in primary dentition[J]. BMC GENOMICS,2016,17(X):e558.
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| APA |
Li YH.,Zou CG.,Liu B.,Zhang ZG[*].,Liu J[*].,...&Zhou Q.(2016).Oral microbial community typing of caries and pigment in primary dentition.BMC GENOMICS,17(X),e558.
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| MLA |
Li YH,et al."Oral microbial community typing of caries and pigment in primary dentition".BMC GENOMICS 17.X(2016):e558.
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