RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast
文献类型:期刊论文
作者 | Shin J1; Wallingford MC2; Byron M3; Bossenz M4; Lawrence JB3; Jones SN3; Mager J2; Bach I1,5; Gallant J3; Marcho C2 |
刊名 | NATURE
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出版日期 | 2014 |
卷号 | 511期号:7507页码:86-89 |
通讯作者 | Ingolf.bach@umassmed.edu |
英文摘要 | In female mice, two forms of X-chromosome inactivation (XCI) ensure the selective silencing of female sex chromosomes during mouse embryogenesis. Beginning at the four-cell stage, imprinted XCI (iXCI) exclusively silences the paternal X chromosome. Later, around implantation, epiblast cells of the inner cell mass that give rise to the embryo reactivate the paternal X chromosome and undergo a random form of XCI (rXCI)(1,2). Xist, a long non-coding RNA crucial for both forms of XCI, is activated by the ubiquitin ligase RLIM (also known as Rnf12)(3-5). Although RLIM is required for triggering iXCI in mice, its importance for rXCI has been controversial. Here we show that RLIM levels are downregulated in embryonic cells undergoing rXCI. Using mouse genetics we demonstrate that female cells lacking RLIM from pre-implantation stages onwards show hallmarks of XCI, including Xist clouds and H3K27me3 foci, and have full embryogenic potential. These results provide evidence that RLIM is dispensable for rXCI, indicating that in mice an RLIM-independent mechanism activates Xist in the embryo proper. |
语种 | 英语 |
源URL | [http://159.226.149.26:8080/handle/152453/9387] ![]() |
专题 | 昆明动物研究所_科研共享资源 |
作者单位 | 1.Program in Gene Function and Expression, University of Massachusetts Medical School (UMMS), Worcester, Massachusetts 01605, USA 2.Veterinary & Animal Sciences, University of Massachusetts Amherst,Amherst,Massachusetts01003,USA 3.DepartmentofCellandDevelopmentalBiology,UMMS,Worcester,Massachusetts01605,USA 4.OrtenauKlinikumLahr-Ettenheim,Institutfu ¨rPathologie, 77933 Lahr, Germany 5.Program in Molecular Medicine, UMMS, Worcester, Massachusetts 01605, USA |
推荐引用方式 GB/T 7714 | Shin J,Wallingford MC,Byron M,et al. RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast[J]. NATURE,2014,511(7507):86-89. |
APA | Shin J.,Wallingford MC.,Byron M.,Bossenz M.,Lawrence JB.,...&Jiao BW.(2014).RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast.NATURE,511(7507),86-89. |
MLA | Shin J,et al."RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast".NATURE 511.7507(2014):86-89. |
入库方式: OAI收割
来源:昆明动物研究所
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