Thiazolidine reacts with thioreactive biomolecules
文献类型：期刊论文
| 作者 | Su DY1,2; Nian Y1; Zhou M1,4; Yang J[*]1,5; Wang S[*]1,2; Zhang FL1; Hu JS1,2; Cui JM1,3 |
| 刊名 | Free Radical Biology and Medicine
 |
| 出版日期 | 2017 |
| 卷号 | 104期号:X页码:272-279 |
| 关键词 | Glutathione TRPA1 channel Thiazolidine |
| 通讯作者 | jy160@columbia.edu, jianyang@mail.kiz.ac.cn ; wangshu@mail.kiz.ac.cn |
| 英文摘要 | The thiazolidine ring is a biologically active chemical structure and is associated with many pharmacological activities. However, the biological molecules that can interact with the thiazolidine ring are not known. We show that thiazolidine causes sustained activation of the TRPA1 channel and chemically reacts with glutathione, and the chemical reactivity of thiazolidine ring is required for TRPA1 activation. Reducing agents reverse thiazolidine-induced TRPA1 activation, and mutagenesis studies show that nucleophilic cysteine residues in TRPA1 are critical, suggesting an activation mechanism involving thioreactive chemical reactions. In vivo studies show that thiazolidine induces acute pain and inflammation in mouse and these responses are specifically dependent on TRPA1. These results indicate that thiazolidine compounds can chemically react with biological molecules containing nucleophilic cysteines, thereby exerting biological activities. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Natural Science Foundation of China (81302865), Yunnan Applied Basic Research Projects (2013FB074), Youth Innovation Promotion Association of the Chinese Academy of Sciences, Key Research Program of the Chinese Academy of Sciences (KJZD-EW-L03) and West Light Foundation of the Chinese Academy of Sciences to Shu Wang; grants from the National Basic Research Program of China (2014CB910301), Top Talents Program of Yunnan Province (2011HA012), High-level Overseas Talents of Yunnan Province, Yunnan Major Science and Technology Project (2015ZJ002) and the National Natural Science Foundation of China (31370821) to Jian Yang; Grants from the High-level Overseas Talents of Yunnan Province and the National Basic Research Program of China (2014CB910301) to Ming Zhou; and a Grant from High-level Overseas Talents of Yunnan Province to Jianmin Cui. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10831]  |
| 专题 | 昆明动物研究所_离子通道药物研发中心 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204, China 3.Department of Biomedical Engineering, Center for the Investigation of Membrane Excitability Disorders, Cardiac Bioelectricity and Arrhythmia Center, Washington University, St. Louis, MO 63130, USA 4.Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, US 5.Department of Biological Sciences, Columbia University, New York, NY 10027, USA |
| 推荐引用方式 GB/T 7714 | Su DY,Nian Y,Zhou M,et al. Thiazolidine reacts with thioreactive biomolecules[J]. Free Radical Biology and Medicine,2017,104(X):272-279. |
| APA | Su DY.,Nian Y.,Zhou M.,Yang J[*].,Wang S[*].,...&Cui JM.(2017).Thiazolidine reacts with thioreactive biomolecules.Free Radical Biology and Medicine,104(X),272-279. |
| MLA | Su DY,et al."Thiazolidine reacts with thioreactive biomolecules".Free Radical Biology and Medicine 104.X(2017):272-279. |
入库方式： OAI收割
来源：昆明动物研究所
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