用H-Y抗体在宫内处理植入前鼠胚对后代性比的影响
文献类型：期刊论文
| 作者 | 陈云鹤; 周瑆良 |
| 刊名 | 动物学研究
 |
| 出版日期 | 1992 |
| 卷号 | 13期号:2页码:173-176 |
| 关键词 | H-Y抗血清 植入前小鼠胚胎 性比 |
| ISSN号 | 0254-5853 |
| 其他题名 | Effect of Intrauterine Treatment of Mouse Embryos With H-Y Antisera on The Offspring Sex Patio |
| 中文摘要 | 本文以C57BL/6J鼠分2组进行实验。实验组45鼠,对照组20鼠,与同品系雄鼠交配后,在3日龄胚期,通过腹部手术对实验组雌鼠每侧子宫腔内注入未稀释的H-Y抗血清与豚鼠补体(二者以1:2混合)3μl。抗血清是由C57BL/6L雌鼠用同品系雄鼠的脾细胞以2.5×10~7个/只的剂量,每周免疫一次,共免疫7次后制备。对照组的处理操作与实验组相同,但每侧子宫腔内仅注入补体3μl,未注入抗体。实验组中有42只产仔247只(每窝平均6只),雌雄比为2.7(180/67)雌性占73％;对照组20只均产仔共163只(每窝平均8只),雌雄比为0.92(78/85),雌性仔鼠占48％。实验组雌性仔鼠百分率比对照组提高了25％。据X~2检验结果表明,实验组与对照组间的差异很显著(P<0.001). |
| 英文摘要 | Forty-five C57BL/6J female mice were mated with the same strain male.3 μl mixture of undiluted H-Y antisera and guinea pig complement (1∶2) were injectcd into each uterine horn of the female mice,while into each uterine horn of control female mice only 3 μl complement was injected.In consequence,247 offsprings were obtained from 42 of 45 experimental female mice:each litter has 6 offsprings on average,the sex ratio was 2.7 (180♀/67♂),and its percentage was 73%;163 offsprings were obtained from 20 contrel femal;mice:the average of each litter was 8 offsprings,the sex ratio was 0.92(78♀/85♂),and its percentage was 48%.Obviously,the percentage of the fcrmer was 25% higher than that of the latter.The difference betwecn them was very significant (P<0.001). |
| 收录类别 | 其他 |
| 资助信息 | 国家自然科学基金;国家“863”计划生物技术领域研究经费资助项目 |
| 原文出处 | 19921302173.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3253]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 中国科学院昆明动物研究所,昆明650107 |
| 推荐引用方式 GB/T 7714 | 陈云鹤,周瑆良. 用H-Y抗体在宫内处理植入前鼠胚对后代性比的影响[J]. 动物学研究,1992,13(2):173-176. |
| APA | 陈云鹤,&周瑆良.(1992).用H-Y抗体在宫内处理植入前鼠胚对后代性比的影响.动物学研究,13(2),173-176. |
| MLA | 陈云鹤,et al."用H-Y抗体在宫内处理植入前鼠胚对后代性比的影响".动物学研究 13.2(1992):173-176. |
入库方式： OAI收割
来源：昆明动物研究所
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