A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom
文献类型：期刊论文
| 作者 | Li YS[1]1; Liu KF[1]1; Wang QC[1]*1; Ran YL[2]2; Tu GC[2]2 |
| 刊名 | TOXICON
 |
| 出版日期 | 1985 |
| 卷号 | 23期号:6页码:859-903 |
| ISSN号 | 0041-0101 |
| 合作状况 | 其它 |
| 英文摘要 | Y.-S. Li, K.-F. Liu, Q.-C. Wang, Y.-L. Rpan and G.-C. Tu. A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom. Toxicon23, 895–903, 1985. — By means of CM-Sephadex C-50 column chromatography and gel filtration on Sephadex G — 75, a potent platelet function inhibitor was purified from Vipera russelli siamensis venom. It appeared as a single protein band on polyacrylamide gel electrophoresis in the presence or absence of SDS, and consists of 123 amino acid residues. Its NH3-terminal residue is serine. It showed the following characteristics: molecular weight, 13,800; isoelectric point, 10.4; ld(In50), 0.5 ± 0.12 mg/kg (i.v.). The platelet inhibitor exhibited phospholipase A2 activity with a specific activity of 35 μmoles/min/mg. From 2 g of the venom, 70 mg of the purified inhibitor was obtained. Inhibition of human platelet aggregation induced by ADP or adrenaline was dose-dependent, with id(In50) of 1.14μg/ml or 0.37 μg/ml, respectively. The platelet aggregation induced by thrombin or collagen was also inhibited and the inhibitory activity on platelet aggregation was heat stable (at 100°C, 20 min) in an acidic medium (pH 5.8), while its phospholipase A2 activity was relatively heat labile under the same condition. The release of 3H-serotonin in platelets stimulated by ADP was also inhibited and this was positively correlated with inhibition of platelet aegregation induced by ADP (r = 0.998, P < 0.002). |
| 收录类别 | SCI |
| 原文出处 | 198523895.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/4991]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Pharmacology, Fujian Medical College, Fuzhou, People's Republic of China 2.Kunming Institute of Zoology, Academia Sinica, Kunming, People's Republic of China |
| 推荐引用方式 GB/T 7714 | Li YS[1],Liu KF[1],Wang QC[1]*,et al. A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom[J]. TOXICON,1985,23(6):859-903. |
| APA | Li YS[1],Liu KF[1],Wang QC[1]*,Ran YL[2],&Tu GC[2].(1985).A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom.TOXICON,23(6),859-903. |
| MLA | Li YS[1],et al."A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom".TOXICON 23.6(1985):859-903. |
入库方式： OAI收割
来源：昆明动物研究所
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