Separation and purification of ovulation-inducing factors in the seminal plasma of the Bactrian camel (Camelus bactrianus)
文献类型：期刊论文
| 作者 | Li XL[*]1,2; Zhao XX1 |
| 刊名 | VETERINARY RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2004 |
| 卷号 | 28期号:3页码:235-245 |
| 关键词 | Bactrian camel follicle follicle-stimulating hormone luteinizing hormone seminal plasma |
| ISSN号 | 0165-7380 |
| 通讯作者 | lixilong@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Ovulation in the Bactrian camel depends upon ovulation-inducing factors in the seminal plasma. The present study was conducted to isolate and purify the bioactive fractions from the seminal plasma of these camels. The seminal plasma was fractionated by anion-exchange chromatography, and six fractions were obtained. The bioactive potential of each fraction was estimated from its effect on rat pituitary tissue cultured in vitro and by the effect of an intramuscular injection of the fraction into female camels in vivo. Both the third fraction (F3) and the fifth fraction (F5) stimulated the release of LH in vitro and in vivo. In addition, female camels ovulated within 48 h after intramuscular injection of F3. However, neither F3 nor F5 had any significant effect on the secretion of FSH, either in vitro or in vivo. When F3 was further fractionated into four subfractions, the third subfraction (F3-3) still stimulated the in vitro release of LH, but not of FSH. An attempt to further purify the ovulation-inducing factors in F3-3 failed owing to the similarity of the molecular characters. |
| 收录类别 | SCI |
| 原文出处 | 200428235.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5333]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Veterinary MedicineGansu Agricultural UniversityLanzhou, GansuP.R.China 2.Kunming Institute of ZoologyChinese Academy of SciencesKunming, YunnanP.R.China |
| 推荐引用方式 GB/T 7714 | Li XL[*],Zhao XX. Separation and purification of ovulation-inducing factors in the seminal plasma of the Bactrian camel (Camelus bactrianus)[J]. VETERINARY RESEARCH COMMUNICATIONS,2004,28(3):235-245. |
| APA | Li XL[*],&Zhao XX.(2004).Separation and purification of ovulation-inducing factors in the seminal plasma of the Bactrian camel (Camelus bactrianus).VETERINARY RESEARCH COMMUNICATIONS,28(3),235-245. |
| MLA | Li XL[*],et al."Separation and purification of ovulation-inducing factors in the seminal plasma of the Bactrian camel (Camelus bactrianus)".VETERINARY RESEARCH COMMUNICATIONS 28.3(2004):235-245. |
入库方式： OAI收割
来源：昆明动物研究所
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