Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori
文献类型：期刊论文
| 作者 | Xu HF1; Liu YW1,2; Wang F1; Yuan L1; Wang YC1; Ma SY1; Benes H[*]3; Xia QY[*]1 |
| 刊名 | TRANSGENIC RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 23期号:4页码:669-677 |
| 关键词 | Bmlp3 promoter Fat body Transgenic silkworm Overexpression Phytase gene |
| 通讯作者 | beneshelen@uams.edu |
| 中文摘要 | In a previous study, we isolated 1,119 bp of upstream promoter sequence from Bmlp3, a gene encoding a member of the silkworm 30 K storage protein family, and demonstrated that it was sufficient to direct fat body-specific expression of a reporter gene in a transgenic silkworm, thus highlighting the potential use of this promoter for both functional genomics research and biotechnology applications. To test whether the Bmlp3 promoter can be used to produce recombinant proteins in the fat body of silkworm pupae, we generated a transgenic line of Bombyx mori which harbors a codon-optimized Aspergillus niger phytase gene (phyA) under the control of the Bmlp3 promoter. Here we show that the Bmlp3 promoter drives high levels of phyA expression in the fat body, and that the recombinant phyA protein is highly active (99.05 and 54.80 U/g in fat body extracts and fresh pupa, respectively). We also show that the recombinant phyA has two optimum pH ranges (1.5-2.0 and 5.5-6.0), and two optimum temperatures (55 and 37 A degrees C). The activity of recombinant phyA was lost after high-temperature drying, but treating with boiling water was less harmful, its residual activity was approximately 84 % of the level observed in untreated samples. These results offer an opportunity not only for better utilization of large amounts of silkworm pupae generated during silk production, but also provide a novel method for mass production of low-cost recombinant phytase using transgenic silkworms |
| 资助信息 | This work was supported by the Grant (No. 2012CB114600) from the National Basic Research Program of China, Grant (No. 31000981) from National Natural Science Foundation of China, and Grant (No. CSTC2010BB1144) from the Natural Science Foundation of Chongqing. |
| 语种 | 英语 |
| WOS记录号 | WOS:000337094700010 |
| 公开日期 | 2014-10-10 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8027]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Department of Neurobiology and Developmental Sciences, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham St., #510, Little Rock, AR 72205, USA |
| 推荐引用方式 GB/T 7714 | Xu HF,Liu YW,Wang F,et al. Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori[J]. TRANSGENIC RESEARCH,2014,23(4):669-677. |
| APA | Xu HF.,Liu YW.,Wang F.,Yuan L.,Wang YC.,...&Xia QY[*].(2014).Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori.TRANSGENIC RESEARCH,23(4),669-677. |
| MLA | Xu HF,et al."Overexpression and functional characterization of an Aspergillus niger phytase in the fat body of transgenic silkworm, Bombyx mori".TRANSGENIC RESEARCH 23.4(2014):669-677. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。