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Introgression from Domestic Goat Generated Variation at the Major Histocompatibility Complex of Alpine Ibex
文献类型：期刊论文
| 作者 | Grossen C[*]1,2; Keller L1; Biebach I1; The International Goat Genome Consortium3,4 |
| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2014 |
| 卷号 | 10期号:6页码:e1004438 |
| 通讯作者 | christine.grossen@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | The major histocompatibility complex (MHC) is a crucial component of the vertebrate immune system and shows extremely high levels of genetic polymorphism. The extraordinary genetic variation is thought to be ancient polymorphisms maintained by balancing selection. However, introgression from related species was recently proposed as an additional mechanism. Here we provide evidence for introgression at the MHC in Alpine ibex (Capra ibex ibex). At a usually very polymorphic MHC exon involved in pathogen recognition (DRB exon 2), Alpine ibex carried only two alleles. We found that one of these DRB alleles is identical to a DRB allele of domestic goats (Capra aegagrus hircus). We sequenced 2489 bp of the coding and non-coding regions of the DRB gene and found that Alpine ibex homozygous for the goat-type DRB exon 2 allele showed nearly identical sequences (99.8%) to a breed of domestic goats. Using Sanger and RAD sequencing, microsatellite and SNP chip data, we show that the chromosomal region containing the goat-type DRB allele has a signature of recent introgression in Alpine ibex. A region of approximately 750 kb including the DRB locus showed high rates of heterozygosity in individuals carrying one copy of the goat-type DRB allele. These individuals shared SNP alleles both with domestic goats and other Alpine ibex. In a survey of four Alpine ibex populations, we found that the region surrounding the DRB allele shows strong linkage disequilibria, strong sequence clustering and low diversity among haplotypes carrying the goat-type allele. Introgression at the MHC is likely adaptive and introgression critically increased MHC DRB diversity in the genetically impoverished Alpine ibex. Our finding contradicts the long-standing view that genetic variability at the MHC is solely a consequence of ancient trans-species polymorphism. Introgression is likely an underappreciated source of genetic diversity at the MHC and other loci under balancing selection. |
| 资助信息 | This work was supported by Swiss National Science Foundation grants (http://www.snf.ch) to CG [PBLAP3-14584] and DC [PA00P3_145360]. The Swiss Federal Office for the Environment and the University of Zurich provided funding to IB and LK. |
| 语种 | 英语 |
| WOS记录号 | WOS:000338847700052 |
| 公开日期 | 2014-10-15 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8040]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Institute of Evolutionary Biology and Environmental Studies, University of Zu ¨rich, Zu ¨rich, Switzerland 2.Department of Zoology, University of British Columbia, Vancouver, Canada 3.Kunming Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Genetic Resources and Evolution, Kunming, China 4.INRA, UMR444, Laboratoire de Genetique Cellulaire, Castanet-Tolosan, France 5.Michael Smith Laboratories, University of British Columbia, Vancouver, Canada |
| 推荐引用方式 GB/T 7714 | Grossen C[*],Keller L,Biebach I,et al. Introgression from Domestic Goat Generated Variation at the Major Histocompatibility Complex of Alpine Ibex[J]. PLOS GENETICS,2014,10(6):e1004438. |
| APA | Grossen C[*],Keller L,Biebach I,&The International Goat Genome Consortium.(2014).Introgression from Domestic Goat Generated Variation at the Major Histocompatibility Complex of Alpine Ibex.PLOS GENETICS,10(6),e1004438. |
| MLA | Grossen C[*],et al."Introgression from Domestic Goat Generated Variation at the Major Histocompatibility Complex of Alpine Ibex".PLOS GENETICS 10.6(2014):e1004438. |
入库方式： OAI收割
来源：昆明动物研究所
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